Community Composition and Diversity Analysis of Rhizosphere Soil Fungi and Endophytic Fungi in Different Tissues of Alfalfa
The community composition,core endophytic fungi genera,Alpha and Beta diversity and correlation networks analysis of endophytic fungi in roots,stems,leaves and rhizosphere soil fungi of alfalfa were analyzed by ITS amplicon sequencing technology.The results showed that the fungal community in rhizosphere soil and the endophytic fungi in roots,stems,leaves of alfalfa belonged to 13 phyla,176 fami-lies,348 genera and 498 species,and there were certain differences in fungal community composition and diversity in different parts.Among them,rhizosphere soil fungi were the most abundant,ACE index,Chao index and Shannon index of alpha diversity were significantly higher than those of plant tissues,Simp-son index was significantly lower than those of plant tissues,the predominant fungal genera were Gibber-ella,unclassified_f_Didymellaceae,Mortierella,Beauveria and Lectera.Beta diversity analysis showed significant differences in the composition of fungal communities in the rhizosphere soil and endophytic fungi in different parts of alfalfa.At the genus level,the dominant endophytic fungi in the roots were unclassi-fied_f_Didymellaceae,Cladosporium,Filobasidium,Titaea and unclassified_f_Dipodascaceae.The dominant endophytic fungal genera in the stems were Filobasidium,unclassified_f_Didymellaceae,Asco-chyta,Vishniacozyma and Cladosporium.The dominant endophytic fungi in the leaves were unclassi-fied_f_Didymellaceae,Ascochyta,Filobasidium,Apiotrichum and Cladosporium.Furthermore,the core fungal genera endophytes of endophytic fungi in roots,stems,and leaves of alfalfa were mainly composed of unclassified_f_Dipodascaceae,Filobasidium,Cladosporium,Ascochyta,Apiotrichum and Vishniaco-zyma.Some fungi can enter the root system from the rhizosphere soil for colonization and then migrate upwards.Additionally,there were a significant interaction between the endophytic fungi in different tissues and fungal community in the rhizosphere soil of alfalfa,with a positive correlation dominant,accounting for 66.56%.
AlfalfaEndophytic fungiFungal diversityDominant genera
NETL
NSTL
万方数据
