褪黑素浸种对干旱胁迫下白三叶种子萌发和幼苗生长的影响
Effects of Melatonin Soaking on Seed Germination and Seedling Growth of Trifolium repens under Drought Stress
熊海琳 1田小霞 2毛培春 2郑明利 2孟林 2付娟娟3
作者信息
- 1. 西北农林科技大学草业与草原学院,陕西 杨凌 712100;北京市农林科学院草业花卉与景观生态研究所,北京 100097
- 2. 北京市农林科学院草业花卉与景观生态研究所,北京 100097
- 3. 西北农林科技大学草业与草原学院,陕西 杨凌 712100
- 折叠
摘要
本研究以'Haifa'白三叶(Trifolium repens'Haifa')为试验材料,探讨了干旱(15%PEG-6000溶液)胁迫下不同浓度(50,100,150,200,250 μmol/L)褪黑素(MT)浸种对其种子萌发,幼苗生长与生理特性的影响.结果表明,PEG胁迫显著抑制了白三叶种子萌发和幼苗正常生长;采用50～200 μmol/L外源褪黑素浸种均可促进干旱胁迫下白三叶种子萌发,提高干旱胁迫下种子萌发指数,幼苗叶绿素含量、可溶性蛋白含量和SOD活性;且随着褪黑素浓度增加均呈先增后降的趋势.隶属函数分析结果显示,MT浸种对促进干旱胁迫下'Haifa'白三叶种子萌发的综合作用排序为MT150>MT200>MT100>MT50>MT250,最佳处理浓度为 150 μmol/L.综上,褪黑素浸种可显著促进干旱胁迫下'Haifa'白三叶种子萌发和幼苗生长,为进一步在干旱半干旱地区应用褪黑素开展白三叶抗旱管理提供理论依据和技术支撑.
Abstract
In this study,Trifolium repens 'Haifa' was used as experimental material to investigate the effects of different melatonin(MT)concentrations(50,100,150,200,250 μmol/L)on the seed germina-tion,seedling growth and physiological characteristics under drought stress(15%PEG-6000 solution).The results showed that PEG stress significantly inhibited the seed germination and seedling growth.Soak-ing seeds with exogenous melatonin from 50 to 200 μmol/L could promote seed germination under drought stress,increase seed germination index,chlorophyll content,soluble protein content and SOD activity of seedlings under drought stress.With the increase of melatonin concentration,the trend was first increased and then decreased.The results of membership function analysis indicated that the comprehensive effect of MT soaking on promoting the germination of 'Haifa' white clover seeds under drought stress were MT150>MT200>MT100>MT50>MT250,and the optimal treatment concentration was 150 μmol/L.In conclu-sion,melatonin soaking can significantly promote seed germination and seedling growth of 'Haifa' white clo-ver under drought stress,and provide a theoretical basis and technical support for further application of MT in drought-resistant management of white clover in arid and semi-arid regions.
关键词
褪黑素/白三叶/干旱胁迫/种子萌发/幼苗生长Key words
Melatonin/Trifolium repens/Drought stress/Seed germination/Seedlings growth引用本文复制引用
基金项目
北京市农林科学院科技创新能力建设专项(KJCX20230109)
国家重点研发计划(2022YFD1300804)
出版年
2024
NETL
NSTL
万方数据