Effects of Melatonin Soaking on Seed Germination and Seedling Growth of Trifolium repens under Drought Stress
In this study,Trifolium repens 'Haifa' was used as experimental material to investigate the effects of different melatonin(MT)concentrations(50,100,150,200,250 μmol/L)on the seed germina-tion,seedling growth and physiological characteristics under drought stress(15%PEG-6000 solution).The results showed that PEG stress significantly inhibited the seed germination and seedling growth.Soak-ing seeds with exogenous melatonin from 50 to 200 μmol/L could promote seed germination under drought stress,increase seed germination index,chlorophyll content,soluble protein content and SOD activity of seedlings under drought stress.With the increase of melatonin concentration,the trend was first increased and then decreased.The results of membership function analysis indicated that the comprehensive effect of MT soaking on promoting the germination of 'Haifa' white clover seeds under drought stress were MT150>MT200>MT100>MT50>MT250,and the optimal treatment concentration was 150 μmol/L.In conclu-sion,melatonin soaking can significantly promote seed germination and seedling growth of 'Haifa' white clo-ver under drought stress,and provide a theoretical basis and technical support for further application of MT in drought-resistant management of white clover in arid and semi-arid regions.
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