Effects of Sucrose Addition on the Archaeal and Methanogenic Bacterial Communities in Alfalfa Silage
Metagenomic sequencing was employed to investigate the impact of 2%sucrose supplemen-tation on the diversity of archaeal and methanogenic bacterial communities in high-moisture alfalfa silage compared to a control group without sucrose addition.The results showed that the control group had infe-rior fermentation quality,while sucrose addition significantly increased lactate content(P<0.05)and improved overall fermentation quality.Nitrososphaera gargensis was the most abundant archaeal species on the raw alfalfa material.Methanosarcina mazei emerged as the dominant archaeal species in the control group,while N.gargensis became dominant species in the sucrose-treated group.The phnJ gene was iden-tified as a putative functional marker for methane synthesis in alfalfa silage.Notably,Escherichia coli and Klebsiella pneumoniae were identified as the dominant methanogenic bacteria in both treatments.How-ever,sucrose addition significantly reduced the relative abundance of K.pneumoniae.Interestingly,a posi-tive correlation was observed between the relative abundance of M.mazei and the levels of butyric acid and ammonia nitrogen.In conclusion,our findings suggest that M.mazei dominates the archaeal community in alfalfa silage,while Enterobacteriaceae family members likely represent the primary methanogenic bacte-ria.Sucrose supplementation appears to partially inhibit the proliferation of both M.mazei and Enterobacte-riaceae bacteria,leading to a significant improvement in the fermentation quality of high-moisture alfalfa silage.
NETL
NSTL
万方数据
