In order to clarify the main pathogens of alfalfa root rot disease in Inner Mongolia,samples of alfalfa root rot disease were collected from 12 sites in 5 cities of Inner Mongolia.The pathogens were iso-lated and purified using conventional tissue separation methods.The isolates were identified by morphol-ogy combined with ITS and EF-1α gene sequence,and the pathogenicity was determined according to Koch's rule.The results showed that 425 fungi of 17 species of 5 genera were isolated from 12 samples in 5 cities.They were Fusarium acuminatum,F.solani,F.equiseti,F.incarnatum,F.oxysporum,F.avenaceum,F.verticillioides,F.proliferatum,F.falciforme,F.neocosmosporiellum,F.tricinctum,F.virguliforme,F.redolens,Rhizoctonia solani,Plectosphaerella cucumerina,Phoma medicaginis and Paraphoma radicina,in which alfalfa root rot caused by F.verticilliforme,F.falciforme,F.neocosmos-poriellum and F.virguliforme was first reported worldwide.The pathogenicity test showed that 17 kinds of fungi could cause alfalfa root rot.Alfalfa root rot disease could be caused by a single pathogen or a combi-nation of 2-4 pathogens,and the pathogenic population structure was different in different regions,but the dominant pathogenic species was F.acuminatum.
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