Identification of Pathogen of Alfalfa Root Rot in Inner Mongolia
In order to clarify the main pathogens of alfalfa root rot disease in Inner Mongolia,samples of alfalfa root rot disease were collected from 12 sites in 5 cities of Inner Mongolia.The pathogens were iso-lated and purified using conventional tissue separation methods.The isolates were identified by morphol-ogy combined with ITS and EF-1α gene sequence,and the pathogenicity was determined according to Koch's rule.The results showed that 425 fungi of 17 species of 5 genera were isolated from 12 samples in 5 cities.They were Fusarium acuminatum,F.solani,F.equiseti,F.incarnatum,F.oxysporum,F.avenaceum,F.verticillioides,F.proliferatum,F.falciforme,F.neocosmosporiellum,F.tricinctum,F.virguliforme,F.redolens,Rhizoctonia solani,Plectosphaerella cucumerina,Phoma medicaginis and Paraphoma radicina,in which alfalfa root rot caused by F.verticilliforme,F.falciforme,F.neocosmos-poriellum and F.virguliforme was first reported worldwide.The pathogenicity test showed that 17 kinds of fungi could cause alfalfa root rot.Alfalfa root rot disease could be caused by a single pathogen or a combi-nation of 2-4 pathogens,and the pathogenic population structure was different in different regions,but the dominant pathogenic species was F.acuminatum.
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