Effects of Sucrose Addition on Functional Genes and Microbial Communities in the Carbon Cycle of Alfalfa Silage
Metagenomic sequencing was employed to investigate the effects of sucrose addition(SG group)compared to no addition(CG group)on carbon transformation,functional genes,and microbial communities within alfalfa silage.The results showed that silage quality was inferior in the CG group,whereas the SG group exhibited a significant higher lactic acid content(P<0.05),indicating improved fer-mentation.Organic carbon oxidation and fermentation were identified as the key steps in the alfalfa silage carbon cycle.In the CG group,Citrobacter freundii,Enterobacter cloacae,and Escherichia coli were prominent contributors to both organic carbon oxidation and fermentation.In contrast,the SG group dis-played a dominant role for Lactobacillus plantarum,Enterobacter cloacae,and Escherichia coli.The fdoG gene was identified as the primary functional gene for organic carbon oxidation,primarily harbored by Citro-bacter freundii and Escherichia coli.The pflD gene was recognized as the main functional gene for fermen-tation,mainly hosted by Enterobacter cloacae and Escherichia coli.These findings suggest Enterobacteria-ceae as the key functional taxa involved in organic carbon oxidation and fermentation in alfalfa silage.Nota-bly,sucrose addition amplified the contribution of Lactobacillus plantarum to both organic carbon oxidation and fermentation processes.
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