添加蔗糖对苜蓿青贮碳循环中功能基因及菌群的影响
Effects of Sucrose Addition on Functional Genes and Microbial Communities in the Carbon Cycle of Alfalfa Silage
李荣荣 1胡明 1魏睿 1蔡瑞2
作者信息
- 1. 渭南师范学院环境与生命科学学院,陕西 渭南 714000
- 2. 华中科技大学环境科学与工程学院,湖北 武汉 430074
- 折叠
摘要
本研究采用宏基因组学技术分析不添加蔗糖(CG组)和添加蔗糖(SG组)对苜蓿青贮中碳素转化、功能基因和微生物的影响.结果表明:CG组青贮品质较差,而SG组显著提高了乳酸含量(P<0.05),改善了发酵品质.有机碳氧化和发酵是苜蓿青贮中主要的碳循环步骤,弗氏柠檬杆菌(Citrobacter freundii)、阴沟肠杆菌(Enterobacter cloacae)和大肠杆菌(Escherichia coli)是CG组中参与有机碳氧化和发酵的优势微生物,而植物乳杆菌(Lactobacillus plantarum)、阴沟肠杆菌和大肠杆菌是SG组中的优势微生物.fdoG是苜蓿青贮中有机碳氧化步骤中的主要功能基因,弗氏柠檬酸杆菌和大肠杆菌是优势宿主.pflD是发酵步骤中的主要功能基因,阴沟肠杆菌和大肠杆菌是优势宿主.因此,肠杆菌科细菌是苜蓿青贮中参与有机碳氧化和发酵步骤的主要功能微生物,而蔗糖添加剂增强了植物乳杆菌在有机碳氧化和发酵步骤中的作用.
Abstract
Metagenomic sequencing was employed to investigate the effects of sucrose addition(SG group)compared to no addition(CG group)on carbon transformation,functional genes,and microbial communities within alfalfa silage.The results showed that silage quality was inferior in the CG group,whereas the SG group exhibited a significant higher lactic acid content(P<0.05),indicating improved fer-mentation.Organic carbon oxidation and fermentation were identified as the key steps in the alfalfa silage carbon cycle.In the CG group,Citrobacter freundii,Enterobacter cloacae,and Escherichia coli were prominent contributors to both organic carbon oxidation and fermentation.In contrast,the SG group dis-played a dominant role for Lactobacillus plantarum,Enterobacter cloacae,and Escherichia coli.The fdoG gene was identified as the primary functional gene for organic carbon oxidation,primarily harbored by Citro-bacter freundii and Escherichia coli.The pflD gene was recognized as the main functional gene for fermen-tation,mainly hosted by Enterobacter cloacae and Escherichia coli.These findings suggest Enterobacteria-ceae as the key functional taxa involved in organic carbon oxidation and fermentation in alfalfa silage.Nota-bly,sucrose addition amplified the contribution of Lactobacillus plantarum to both organic carbon oxidation and fermentation processes.
关键词
苜蓿青贮/高水分/宏基因组/碳循环Key words
Alfalfa silage/High moisture/Metagenomics/Carbon cycle引用本文复制引用
基金项目
国家自然科学基金(32302796)
渭南师范学院人才项目(2022RC24)
出版年
2024
NETL
NSTL
万方数据