摘要
本研究对燕麦低氮不敏感材料As581和低氮敏感材料As533幼苗(3叶1心期)进行低氮胁迫处理,于胁迫处理12 h采集根系和叶片进行转录组测序,以期揭示燕麦低氮胁迫相关的分子及信号通路,同时为低氮胁迫响应候选基因的克隆与功能验证奠定基础.结果表明,与As581相比,低氮条件下敏感材料As533株高、叶绿素含量、氮含量、叶长、叶宽、叶面积、根冠比、最长主根长和根面积的差异更明显.低氮条件下,As581中有466个差异表达基因,As533中有1090个差异表达基因,两个材料叶片中的差异表达基因均比根系多,且多为下调.GO分析表明,As533和As581根系和叶片生物学过程都富集到代谢过程,细胞组分都富集到细胞和细胞组分,分子功能都富集到催化活性、结合和转运活性.KEGG分析表明,As533根系和叶片都富集在苯丙氨酸代谢、植物昼夜节律、淀粉和蔗糖代谢通路,As581根系和叶片都富集在氮代谢、ABC转运、TCA循环、氧化磷酸化以及丙氨酸、天冬氨酸和谷氨酸代谢通路中.RT-qPCR分析表明,6个低氮相关差异表达基因的表达量变化趋势与RNA-seq结果一致.
Abstract
In this study,oat seedlings(3 leaves 1 heart stage)of low nitrogen insensitive material As581 and low-nitrogen sensitive material As533 were treated with low nitrogen stress.After 12 h of stress treatment,roots and leaves were collected for transcriptome sequencing,in order to reveal molecular and signaling pathways related to low-nitrogen stress in oat,and lay a foundation for cloning and functional verification of candidate genes.The results showed that compared with As581,the sensitive material As533 had more significant differences in plant height,chlorophyll content,nitrogen content,leaf length,leaf width,leaf area,root shoot ratio,longest main root length,and root area under low nitrogen conditions.Under low nitrogen conditions,there were 466 differentially expressed genes in oat As581 and 1090 in oat As533.The differentially expressed genes in leaves of the two materi-als were more than those in roots,and most of them were down-regulated.GO analysis showed that both the root and leaf biological processes of As533 and As581 were enriched in metabolic processes,cell components were enriched in cell and cell components,and molecular functions were enriched in catalytic activities,binding and trans-port activities.KEGG analysis showed that As533 roots and leaves were enriched in phenylalanine metabolism,plant circadian rhythm and starch and sucrose metabolism pathways,while As581 roots and leaves were enriched in nitrogen metabolism,ABC transport,TCA cycle,metabolism of alanine,aspartic acid and glutamate,and oxida-tive phosphorylation pathways.RT-qPCR analysis of candidate genes associated to response to low nitrogen stress showed that the expression trend of differentially expressed genes was consistent with that of RNA-seq.
基金项目
内蒙古科技厅中央引导地方科技发展资金项目(2022ZY0047)
内蒙古农业大学生命科学学院团队发展基金(TD202103)
麦类种质创新利用自治区高等学校重点实验室支持()
NETL
NSTL
万方数据