Phenotypic and Transcriptome Analysis of Oat Seedlings in Response to Low Nitrogen Stress
In this study,oat seedlings(3 leaves 1 heart stage)of low nitrogen insensitive material As581 and low-nitrogen sensitive material As533 were treated with low nitrogen stress.After 12 h of stress treatment,roots and leaves were collected for transcriptome sequencing,in order to reveal molecular and signaling pathways related to low-nitrogen stress in oat,and lay a foundation for cloning and functional verification of candidate genes.The results showed that compared with As581,the sensitive material As533 had more significant differences in plant height,chlorophyll content,nitrogen content,leaf length,leaf width,leaf area,root shoot ratio,longest main root length,and root area under low nitrogen conditions.Under low nitrogen conditions,there were 466 differentially expressed genes in oat As581 and 1090 in oat As533.The differentially expressed genes in leaves of the two materi-als were more than those in roots,and most of them were down-regulated.GO analysis showed that both the root and leaf biological processes of As533 and As581 were enriched in metabolic processes,cell components were enriched in cell and cell components,and molecular functions were enriched in catalytic activities,binding and trans-port activities.KEGG analysis showed that As533 roots and leaves were enriched in phenylalanine metabolism,plant circadian rhythm and starch and sucrose metabolism pathways,while As581 roots and leaves were enriched in nitrogen metabolism,ABC transport,TCA cycle,metabolism of alanine,aspartic acid and glutamate,and oxida-tive phosphorylation pathways.RT-qPCR analysis of candidate genes associated to response to low nitrogen stress showed that the expression trend of differentially expressed genes was consistent with that of RNA-seq.
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