Construction and Comparison of Induction and Regeneration System for Immature Embryos of Different Genotypes of Elytrigia elongata
Elytrigia elongata is a kind of perennial forage and ecological grass with saline-alkali toler-ance,high yield and good quality,which plays an important role in the improvement of saline-alkali land and ecological environment construction in coastal areas in China.However,its biotechnology system is still immature.In this study,8 germplasm from different sources of Elytrigia elongata were used as experi-mental materials,tissue culture technology was used to construct the young embryo regeneration system,and excellent acceptor materials suitable for constructing genetic transformation system were screened.The results showed that on the modified induction medium(MS medium+0.5 g/L L-glutamic acid+0.5 g/L L-proline+0.3 g/L enzymatically hydrolyzed casein+30 g/L maltose+2 mg/L 2,4-D+7 g/L AGAR),a smaller callus was formed on the third day of Elytrigia elongata germplasm culture,and differentiation occurred after 4 weeks of culture.The callus induction rate,differentiation rate,and regeneration time of young embryo of different genotypes were significantly different on the same medium.Among them,the callus induction rate of young embryos of germplasm No.4 was as high as 97.92%,the differentiation rate was 88.33%,the rooting rate was 100.00%,and the regeneration time was relatively short(56.2 d),which could be used as an ideal chassis material for genetic transformation of Elytrigia elongata.
Elytrigia elongataCallusYoung embryosRegeneration system
NETL
NSTL
万方数据
