Cloning and Expression Analysis of AsSOS1 Gene in Oat under Salt Stress
In order to explore the function of the main salt tolerance gene AsSOS1(Na+/H+antiporter protein)in oat(Avena sativa),the salt-tolerant variety Qingyongjiu 195 was used as the test material,and AsSOS1 gene was cloned after three weeks of seedling cultivation.The protein sequence was homologous aligned and analyzed for physicochemical properties,predicted for protein transmembrane structure and hydrophilicity/hydrophobicity,secondary and tertiary structures.The expression of AsSOS1 gene in oat roots,stems and leaves of oat under 100 mmol/L NaCl stress was analyzed by qRT-PCR.The results indi-cated that the length of open reading frame of AsSOS1 gene was 3411 bp,encoding 1137 amino acids.AsSOS1 protein had a molecular weight of 126.088 KDa and an isoelectric point of 6.62.It was an acid protein with a stability coefficient of 45.14 and hydrophobic protein.The secondary structure of AsSOS1 protein contained 48.20%α-helix and 33.77%random coil,and the tertiary structure was consistent with the predicted secondary structure,mainly α-helix.The results of transmembrane domain prediction showed that AsSOS1 protein may had extracellular domain,transmembrane helical domain and intracellular domain,among which,amino acid sequence 11-424 were conserved sequence of Na+/H+antiporter,indi-cating this region was predicted to be related to plant salt tolerance.Evolutionary analysis found that it was most closely related to Lulium rigidum,with a similarity of up to 96%.Under normal growth conditions,AsSOS1 gene was expressed in oat roots,stems and leaves.Under 100 mmol/L NaCl stress,the expres-sion of AsSOS1 in stem and root increased by 171.4%and 54.1%,respectively.The results indicate that AsSOS1 is induced by salt stress and plays an important role in salt tolerance of oat.
万方数据
维普
