Transcriptomic Analysis of the Development of Apomixis Embryo Sac in Poa pratensis(Kentucky Bluegrass)
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维普
万方数据
为探究草地早熟禾无融合生殖胚囊发育过程中基因表达谱的变化,本研究利用高通量Illu-mina Hiseq测序技术对'清水'草地早熟禾无融合生殖子房发育的无孢子起始细胞发生时期(8DBA)和无孢子生殖胚囊发育时期(6DBA、4DBA和2DBA)共4个阶段的小穗进行转录组测序.结果显示,不同差异分组(2DBA vs 4DBA、2DBA vs 6DBA和2DBA vs 8DBA)共有13575个DEGs,包括7191个上调基因和6384个下调基因.GO富集分析发现,3个差异分组的DEGs显著富集在过氧化氢的响应、蛋白质重折叠、营养水库活动、错误折叠蛋白结合和双糖生物合成等过程中.KEGG通路分析表明,3个差异分组的DEGs显著富集在代谢途径、次生代谢产物的生物合成、淀粉和蔗糖代谢及内质网中的蛋白加工等通路上,在2DBA vs 8DBA分组中发现植物激素信号转导途径的富集.4个时期占比最多的转录因子家族是NAC、AP2/ERF-ERF和MYB-related等.将在 4个发育阶段特异性高表达的基因OPR1、ASN2、SAMS2、HSP81-2、PXG4和FPK2作为与'清水'草地早熟禾无融合生殖胚囊发育相关的候选基因.qRT-PCR分析表明,除WOX2在4DBA上调,与RNA-seq的表达存在差异外,其余16个DEGs的表达量变化趋势与高通量测序结果基本一致.
In this research for changes of gene expression profile in apomixis embryo sac development of Poa pratensis,transcriptomic sequencing analysis was applied on spikelet of Poa pratensis cultivar'Qin-gshui'in four stages of apomixis ovary development,apospore initiation cell stage(8DBA)and apospore embryo sac development stages(6DBA,4DBA,and 2DBA),by Illu-mina Hiseq sequencing technology.Results revealed a difference with 13575 DEGs for differential groups(2DBA vs 4DBA,2DBA vs 6DBA,and 2DBA vs 8DBA),including 7191 up-regulated genes and 6384 down-regulated genes.GO enrichment analysis showed that DEGs of three differential groups significantly clustered in processes of hydrogen per-oxide response,protein refolding,nutrition reservoir activity,misfolded protein binding,and disaccharide biosynthesis.DEGs of three differential groups were found significantly enriched on pathways such as meta-bolic pathways,biosynthesis of secondary metabolites,starch and sucrose metabolism,and protein process-ing in endoplasmic reticulum,from KEGG pathway analysis.Enrichment of plant hormone signal transduc-tion pathway was found in the 2DBA vs 8DBA group.The most parts of TFs in four stages are NAC,AP2/ERF-ERF,and MYB-related,etc.Genes specifically high expressed in four development stages,OPR1,ASN2,SAMS2,HSP81-2,PXG4,and FPK2,were selected as candidates of apomixis embryo sac development in P.pratensis'Qingshui'.qRT-PCR analysis indicated that trend of expression changes in 16 DEGs was consistent with results of high-throughput sequencing,except the up-regulation of WOX2 on 4DBA,whose expression was different to RNA-seq.This study provide evidence for further function research on vital genes related to development of apomixis embryo sac in P.pratensis.
维普
万方数据
