To investigate the effects of complex lactic acid bacteria and moisture content on the fermen-tation of alfalfa silage,alfalfa with water content of 59.50%and 71.40%were used as raw materials.A control group without bacteria(CK),a group of Lactobacillus plantarum+Lactobacillus buchneri(Z+B),a group of Lactobacillus plantarum+Lactobacillus buchneri+Pediococcus pentosaceus(Z+B+W),a group of Lactobacillus plantarum+Lactobacillus buchneri+Lactobacillus kimchi fermentation powder(Z+B+P),a group of Lactobacillus plantarum+Lactobacillus buchneri+Pediococcus pentosaceus+Lac-tobacillus kimchi fermentation powder(Z+B+W+P),and a group of Lactobacillus kimchi fermentation powder(P)were set up for treatment under room temperature conditions.After 60 days of silage fermenta-tion,the quality of alfalfa silage was measured.The results showed that when the water content of alfalfa was 59.50%,the dry matter content and crude protein(CP)content of the group treated with lactic acid bacteria were significantly higher than those of the CK group(P＜0.05),and the pH value was signifi-cantly lower than that of the CK group(P＜0.05);Among them,the Z+B group had the highest dry mat-ter content(38.80%FM),CP content(20.60%DM),and crude fat(EE)content(4.50%DM),the low-est neutral detergent fiber(NDF)content(34.54%DM)and acid detergent fiber(ADF)content(27.50%DM),the lowest pH value(4.55),the highest lactic acid(LA)content(7.40%FM),and no butyric acid(BA)was detected.When the water content of alfalfa was 71.40%,the dry matter and LA content of the group treated with lactic acid bacteria were significantly higher than those of the CK group(P＜0.05),and the pH was significantly lower than that of the CK group(P＜0.05).Among them,the dry matter content(27.55%FM)and CP content(20.30%DM)of the Z+B+W+P group were higher than those of the other groups,the NDF content(36.32%DM)and ADF content(29.25%DM)were the low-est,the LA content(6.79%FM)was the highest,and the BA content(1.09%FM)was the lowest.In summary,the effect of Z+B treatment was the best under low water conditions(59.50%),and the effect of Z+B+W+P treatment on silage was the best under high water conditions(71.40%).
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