Research on the Mining of Salt-Tolerant Related Transcription Factors in Alfalfa Based on Transcriptome Sequencing
In order to identify the transcription factors(TFs)of alfalfa in response to salt stress,salt-tolerant cultivar'Zhongmu No.1'and salt-sensitive cultivar'WL323',were subjected to 200 mmol/L NaCl and the transcriptome data of alfalfa root tissues were analyzed for 0 h,6 h,12 h,and 24 h after stress.The results showed that a total of 758 TFs were differentially expressed in response to salt stress between the two cultivars.GO analysis revealed that differentially expressed TFs(DETFs)were mainly enriched in metabolic processes,binding,and cellular processes.Furthermore,while KEGG significant enrichment analysis showed that these DETFs were enriched in plant hormone signal transduction path-ways.Ten key candidate TFs regulating salt tolerance in alfalfa were identified through comparing the rela-tive expression levels and expression patterns of DETFs in the plant hormone signal transduction path-ways.qRT-PCR analysis showed that the six randomly selected TFs were basically consistent with the results of transcriptome sequencing.
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