摘要
木豆为热带及部分亚热带地区重要的食饲兼用作物,南方低温会导致木豆的减产,开展木豆的耐寒性研究可为耐寒木豆新品种培育提供数据支撑.为探究不同耐寒性木豆的蛋白表达差异特性,本试验以耐低温的缅甸木豆(MD)和低温敏感型的琼中木豆(QZ)种质为材料,4℃低温胁迫96 h后,进行蛋白组测序和DIA分析,结果表明:MD和QZ材料中差异表达蛋白(DEPs)分别为56个和57个;GO分析发现DEPs主要涉及催化活性、结合、细胞过程和代谢过程等,MD中显著富集在氧化还原酶活性、应激反应、信号转导等功能,QZ中显著富集在水解酶活性、水解O-糖基化合物和蓝光光感受体活性等;KEGG分析显示,DEPs涉及代谢通路、次级代谢物生物合成等途径,在MD中显著富集在苯丙烷类生物合成、内质网蛋白加工过程和RNA聚合酶途径,而在QZ中显著富集在氨基糖和核糖代谢、植物昼夜节律和次级代谢物生物合成途径.大部分DEPs亚细胞定位于细胞质、叶绿体和细胞核中.蛋白组学分析表明,过氧化物酶是参与提高木豆耐寒能力的关键蛋白,氧化还原酶活性、应激反应、信号转导、苯丙烷类生物合成、内质网蛋白加工过程等生物学途径参与木豆响应低温胁迫过程.
Abstract
Pigeonpea is an important food and feed crop in the tropical and some subtropical regions.Low temperatures in the southern of China can reduce the yield of pigeonpea.Research on the cold toler-ance characteristics of pigeonpea can provide data support for breeding new varieties of pigeonpea.In order to study the differential protein expression characteristics of cold tolerance pigeonpea,low-temperature tol-erant pigeonpea(Miandian,MD)and low-temperature sensitive pigeonpea(Qiongzhong,QZ)germplasm were used as materials.After 96 h of low temperature stress at 4℃,proteome sequenced and DIA analysis were performed.The results showed that there were 56 and 57 differentially expressed proteins(DEPs)in MD and QZ materials,respectively.GO analysis showed that DEPs were mainly involved in catalytic activ-ity,binding,cellular process and metabolic process.In MD,GO significantly enriched functions such as oxidoreductase activity,stress response,and signal transduction.In QZ,GO significantly enriched hydro-lase activity,hydrolyzing O-glycosyl compounds,blue light photoreceptor activity and others.KEGG analysis showed that DEPs were involved in metabolomic pathway and secondary metabolites biosynthesis.In MD,DEPs were significantly enriched amino sugar and ribose metabolism,plant circadian rhythm,and secondary metabolites biosynthesis.Most DEPs subcellular fraction was localized in cytoplasm,chloro-plasts and nucleus.Peroxidase is the key protein involved to improve the cold tolerance of pigeonpea.The biological process including in oxidoreductase activity,response to stimulus,signal transduction,phenyl-propanoid biosynthesis and protein processing in endoplasmic reticulum and others were involved in the pro-cess response to cold stress in pigeonpea by comparative proteomics analysis.
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