Analysis of Differentially Expressed Proteins in Pigeonpea(Cajanus cajan(L.)Millsp.)Respond to Cold Stress
Pigeonpea is an important food and feed crop in the tropical and some subtropical regions.Low temperatures in the southern of China can reduce the yield of pigeonpea.Research on the cold toler-ance characteristics of pigeonpea can provide data support for breeding new varieties of pigeonpea.In order to study the differential protein expression characteristics of cold tolerance pigeonpea,low-temperature tol-erant pigeonpea(Miandian,MD)and low-temperature sensitive pigeonpea(Qiongzhong,QZ)germplasm were used as materials.After 96 h of low temperature stress at 4℃,proteome sequenced and DIA analysis were performed.The results showed that there were 56 and 57 differentially expressed proteins(DEPs)in MD and QZ materials,respectively.GO analysis showed that DEPs were mainly involved in catalytic activ-ity,binding,cellular process and metabolic process.In MD,GO significantly enriched functions such as oxidoreductase activity,stress response,and signal transduction.In QZ,GO significantly enriched hydro-lase activity,hydrolyzing O-glycosyl compounds,blue light photoreceptor activity and others.KEGG analysis showed that DEPs were involved in metabolomic pathway and secondary metabolites biosynthesis.In MD,DEPs were significantly enriched amino sugar and ribose metabolism,plant circadian rhythm,and secondary metabolites biosynthesis.Most DEPs subcellular fraction was localized in cytoplasm,chloro-plasts and nucleus.Peroxidase is the key protein involved to improve the cold tolerance of pigeonpea.The biological process including in oxidoreductase activity,response to stimulus,signal transduction,phenyl-propanoid biosynthesis and protein processing in endoplasmic reticulum and others were involved in the pro-cess response to cold stress in pigeonpea by comparative proteomics analysis.
Cajanus cajan(L.)MillspLow temperature stressProteomicsDifferentially expressed proteins
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