Effect of PEG Priming on Antioxidant Physiology of Deteriorated Siberian Wildrye Seeds during Imbibition
In this study,siberian wildrye(Elymus sibiricus)seeds were used as materials to compare the germination characteristics and changes in antioxidant indexes during imbibition process(beginning,6 h,72 h,and 144 h)between non-deteriorated(CK)and deteriorated(D14)seeds under controlled dete-rioration and polyethylene glycol(PEG)priming treatments(PEG-5%,PEG-10%,and PEG-20%).Results indicated that compared to CK,the seed germination of D14 was reduced and the seedling growth was inhibited.PEG-10%priming significantly enhanced the germination potential,germination percent-age,germination index,radicle length,seedling length,radicle weight,and seedling weight of deteriorated seeds.Additionally,PEG-10%priming significantly increased the ascorbic acid(AsA)content at begin-ning of imbibition,glutathione(GSH)content at 6 h of imbibition,glutathione reductase(GR)activity at 72 h of imbibition,and peroxidase(POD)activity at 144 h of imbibition,while reduction of hydrogen per-oxide(H2O2)levels at 6 h and 72 h of imbibition and malondialdehyde(MDA)content at 144 h of imbibi-tion were observed.Changings on antioxidant indices can effectively maintain the normal germination pro-cess.In cluster analysis,it was found that the nine antioxidant defense system indicators could be roughly clustered into 3 groups,among which the MDA content,POD activity,and superoxide dismutase(SOD)activity follow the same variation trend at different imbibition times.
Siberian wildryeSeed deteriorationPEG primingAntioxidant system
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