In this study,siberian wildrye(Elymus sibiricus)seeds were used as materials to compare the germination characteristics and changes in antioxidant indexes during imbibition process(beginning,6 h,72 h,and 144 h)between non-deteriorated(CK)and deteriorated(D14)seeds under controlled dete-rioration and polyethylene glycol(PEG)priming treatments(PEG-5%,PEG-10%,and PEG-20%).Results indicated that compared to CK,the seed germination of D14 was reduced and the seedling growth was inhibited.PEG-10%priming significantly enhanced the germination potential,germination percent-age,germination index,radicle length,seedling length,radicle weight,and seedling weight of deteriorated seeds.Additionally,PEG-10%priming significantly increased the ascorbic acid(AsA)content at begin-ning of imbibition,glutathione(GSH)content at 6 h of imbibition,glutathione reductase(GR)activity at 72 h of imbibition,and peroxidase(POD)activity at 144 h of imbibition,while reduction of hydrogen per-oxide(H2O2)levels at 6 h and 72 h of imbibition and malondialdehyde(MDA)content at 144 h of imbibi-tion were observed.Changings on antioxidant indices can effectively maintain the normal germination pro-cess.In cluster analysis,it was found that the nine antioxidant defense system indicators could be roughly clustered into 3 groups,among which the MDA content,POD activity,and superoxide dismutase(SOD)activity follow the same variation trend at different imbibition times.
Siberian wildryeSeed deteriorationPEG primingAntioxidant system
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NSTL
万方数据
