Establishment of HPLC fingerprint and chemometric analysis of Agrimonia pilosa Ledeb
Objective Establish an HPLC fingerprint of Agrimonia pilosa Ledeb.and evaluate the overall quality of Agrimonia pilosa Ledeb.from different regions through chemometric analysis.Methods YMC-Pack ODS-A column(250 mm×4.6 mm,5 μm)was used,mobile phase was acetonitrile-0.1%aqueous phosphoric acid solution,the gradient was eluted with a flow rate of 1.0 ml/min,a column temperature of 30℃,a detection wavelength of 270 nm,sample injection quantity of 10μl.HPLC fingerprint was established and similarity evaluated.15 batches of samples were analyzed by cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(PLS-DA).Results A total of 14 common peaks were identified in the HPLC fingerprint,and 4 components were identified,namely gallic acid in peak 1,catechin in in peak 4,ellagic acid in peak 8,and quercetin in peak 10.The similarity of the fingerprint of 15 batches of Agrimonia pilosa Ledeb.between 0.778 and 0.978 indicates that there are differences in the quality of Agrimonia pilosa Ledeb.from different regions.After CA and PCA analysis,the samples were divided into two categories,and six differential biomarkers were screened using PLS-DA.Conclusion There are certain quality differences in the medicinal materials of Agrimonia pilosa Ledeb.from different sources.The combination of fingerprint,cluster analysis,principal component analysis,and partial least squares discriminant analysis can comprehensively evaluate the quality of Agrimonia pilosa Ledeb..The establishment of this method can provide a certain reference basis for the quality control and product evaluation of Agrimonia pilosa Ledeb..
万方数据
维普
