Study on the systematic quality improvement of Qifeng Tongqiao Mixture in medical institutions
Objective To establish qualitative identification and content determination methods,and provide reference basis for improving the quality standards of Qifeng Tongqiao Mixture.Methods Thin layer chromatography was used to qualitatively identify Astragalus mongholicus,Flos magnoliae,and Pogostemon in the prescription;Using Astragaloside Ⅳ as the reference content,the content of the main medicinal flavor of Astragalus mongholicus in the prescription was determined by high-performance liquid chromatography.Results Under the thin layer chromatography conditions used,the chromatographic spots of Astragalus mongholicus,Flos magnoliae,and Pogostemon were clear,the separation effect was good,and there was no interference;In the HPLC content determination,the linear equation of Astragaloside Ⅳ is y=1.411 8x+4.832 6,and the correlation coefficient R2=0.998 4,indicating that Astragaloside Ⅳ has a good linear in the injection volume relationship in the range of 0.39～3.90 μg.The average recovery rate of this method is 99.78%,and the RSD value is 1.02%(n=9).Conclusion The established qualitative identification and content determination methods are relatively simple,accurate,sensitive,and reproducible,and can be used for the quality control of Qifeng Tongqiao Mixture.
万方数据
维普
