Anti-neuroinflammatory effects of extracts from rhizomes of Atractylodes macrocephala Koidzumi in LPS-induced BV2 microglial cells
Objective The research is to investigate the anti-neuroinflammatory effects and related mechanism of methanol-n-Hexane extracts from rhizomes of Atractylodes macrocephala Koidzumi(AH)in LPS-induced BV2 microglial cells,and analyze the main components of the AH.Methods The extracts from rhizomes of Atractylodes macrocephala Koidzumi were made.The main components of the AH was analyzed by high-performance liquid chromatography(HPLC).BV2 microglial cells were induced by LPS to establish an inflammatory model.MTT assay was used to test the cell viability.The content of NO in cell supernatant was measured by Griess reagent.The levels of PGE2,IL-6,and TNF-α were measured by ELISA kits.The expression of COX-2,iNOS,MAPK and NF-κB proteins were detected by Western blot.Results The main components of AH included atractylenolide Ⅱ and Ⅲ.AH significantly reduced the levels of NO,PGE2,IL-6,and TNF-α produced by LPS-induced BV2 microglial cells,while reduced iNOS,COX-2,MAPK and NF-κ B proteins expression.Additionally,the inhibitory effect of low concentration AH on PGE2 was not weaker than that of high concentration of atractonolide Ⅱ and Ⅲ,and the inhibitory effect of high concentration AH was similar with high concentration atractylenolide Ⅲ.Conclusion The recovery of AH is high,and the main components of AH include atractylenolide Ⅱ and Ⅲ.AH has a better anti-neuroinflammatory effect,and its anti-inflammatory mechanism is related to the MAPK and NF-κB pathways.
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