Isorhamnetin inhibits the proliferation of HepG2 cells by downregulating the Notch1 pathway and induces apoptosis
Objective To investigate whether Isorhamnetin ISO downregulates Notch1 expression to inhibit cell proliferation and promote apoptosis.Methods The cytotoxicity assay(MTT assay)was used to evaluate the viability of HepG2 cells,and flow cytometry was employed to analyze the apoptosis level of the cells.The colony formation assay was performed to assess the proliferation of HepG2 cells under different concentrations of paclitaxel.In addition,Western blot analysis was conducted to determine the expression levels of P53,Bcl-2,and Bax.Results Treatment with ISO significantly suppressed the survival of HCC cells in a dose-and time-dependent manner.Induction of apoptosis was observed in HepG2 cell line 24 hours after ISO treatment.Knockdown of Notch1 expression and upregulation of the anti-cancer gene P53 expression were observed after ISO treatment,as well as upregulation of apoptosis-related protein Bax expression and downregulation of Bcl-2 expression.Further experiments showed that Notch1 siRNA enhanced the anti-tumor properties of ISO.Conclusion ISO inhibits the proliferation of HCC cells and promotes apoptosis by downregulating the Notch1 pathway.
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