Study on the improvement of quality for Shenbao Capsules
Objective To provide reference for the improving of the quality standard for Shenbao Capsules.Methods On the basis of the previous quality standard,thin-layer chromatography(TLC)identification methods were established for trigonella foenum-graecum,astragali radix,red ginseng and poria cocos.High performance liquid chromatography(HPLC)method was established to determine the content of osthol.Results TLC spots were clear,negative without interference.The linear range of the detected mass concentration of osthol is 4.05～121.49 μg/ml(R2= 0.99997).RSD of precision,stability(24 h)and repeatablitity tests were all no more than 0.35%(n= 6).The sample recovery test was divided into high,medium and low groups according to the amount of reference substance added.The average sample recovery rates were 96.05%,96.02%and 96.23%respectively(RSD≤1.97%,n=3).Conclusion The TLC identification methods of trigonella foenum-graecum,astragali radix,red ginseng and poria cocos in Shenbao Capsule and the content determination method of osthol have been established in this study.The methods have strong specificity and good reproducibility,and can be used to improve the quality standard of Shenbao Capsules.
Shenbao CapsulesIdentificationContent determinationImprovement of quality standard
万方数据
维普
