HPLC测定蜈蚣配方颗粒中3,8-二羟基喹啉的含量

Simultaneous determination of quinoline-3,8-diol in Centipede Formula Granules by HPLC

江斌 ¹蔡小兵 ¹梁素仪 ¹李璐 ¹郑晓英 ¹张辉 ¹谭沛¹

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作者信息

1. 华润三九医药股份有限公司,广东深圳 518110;华润三九现代中药制药有限公司,广东惠州 516000
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摘要

目的建立HPLC法测定蜈蚣配方颗粒中 3,8-二羟基喹啉的含量.方法采用Kromasil 100-5 C18 色谱柱(250 mm×4.6 mm,5 μm),以 10 mmol/L的磷酸二氢钾溶液-甲醇(68∶32)为流动相,流速为 1.0 ml/min,柱温为 30℃,检测波长为 252 nm.结果 HPLC色谱图中阴性对照无干扰;3,8-二羟基喹啉在 0.01163～1.395 mg/ml(r= 0.9997)范围内呈良好的线性关系,平均加样回收率为 99.4%(RSD=2.7%).结论该方法准确、稳定、可行,本文建立的方法经方法学验证可用于评价蜈蚣配方颗粒的质量.

Abstract

Objective To establish a HPLC method for simultaneous determination of quinoline-3,8-diol in Centipede Formula Granules.Methods The chromatographic separation was achieved on a Kromasil 100-5 C18(column 250 mm×4.6 mm,5 μm)and the column temperature was at 30℃.The mobile phase consisted of a water solution containing 10 mmol/L monopotassium phosphate and methyl alcohol with isoelution(68∶32)at a flow rate of 1.0 ml/min,and the detection wavelength was set at 252 nm.Results HPLC chromatograms revealed no interference due to negative reference.Quinoline-3,8-diol showed good linear relationships within the ranges of 0.01163～1.395 mg/ml(r= 0.9997),whose average recoveries was 99.4%(RSD=2.7%),respectively.Conclusion This accurate,stable and feasible method can be used for the quality control of Centipede Formula Granules according to the methodology validation.

关键词

蜈蚣配方颗粒/3,8-二羟基喹啉/HPLC

Key words

Centipede Formula Granules/Quinoline-3,8-diol/HPLC

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基金项目

深圳市科技计划(JSGG20191129093418578)

出版年

2024

中国处方药

南方医药经济研究所

中国处方药

影响因子：0.649

ISSN：1671-945X

参考文献量14

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