Simultaneous determination of quinoline-3,8-diol in Centipede Formula Granules by HPLC
Objective To establish a HPLC method for simultaneous determination of quinoline-3,8-diol in Centipede Formula Granules.Methods The chromatographic separation was achieved on a Kromasil 100-5 C18(column 250 mm×4.6 mm,5 μm)and the column temperature was at 30℃.The mobile phase consisted of a water solution containing 10 mmol/L monopotassium phosphate and methyl alcohol with isoelution(68∶32)at a flow rate of 1.0 ml/min,and the detection wavelength was set at 252 nm.Results HPLC chromatograms revealed no interference due to negative reference.Quinoline-3,8-diol showed good linear relationships within the ranges of 0.01163～1.395 mg/ml(r= 0.9997),whose average recoveries was 99.4%(RSD=2.7%),respectively.Conclusion This accurate,stable and feasible method can be used for the quality control of Centipede Formula Granules according to the methodology validation.
万方数据
维普
