Optimization of extracting technology for Shisanwei Zhuyu Mixture by orthogonal test method
Objective To optimize the water extraction decoction process of Shisanwei Zhuyu Mixture for its subsequent study.Methods The number of decoctions(A),water addition(B)and decoction time(C)were used as the investigating factors,and the combined scores of hydroxysafflor yellow A and leachate yield were used as indicators to determine the weighting coefficients of hydroxysafflor yellow A content and leachate yield as 70%and 30%,respectively,to optimize the water extraction decoction process by L9(34)orthogonal test design and to validate the test.Results The most preferred decoction process is to add 8 times the amount of water and decoct 2 times for 90 min each time.Conclusion The preferred process conditions are reasonable,stable and feasible for the standardized extraction process of Shisanwei Zhuyu Mixture.
Shisanwei Zhuyu MixtureOrthogonal test methodHydroxysafflor yellow AExtraction processHPLC
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