Comparison of eight components of Sarcandra glabru in different growth years and different parts
Objective To establish a HPLC method for simultaneous determination of eight components in different growth years and different parts of Sarcandra glabru.Methods The Phenomenex Luna C18(250 mm×4.6 mm,5μm)column was used,the mobile phase was 0.2%phosphoate acid(A)and acetonitrile(B),gradient elution(0～12 min,2%～11%B;12～25 min,11%～18%B;25～40 min,18%～40%B)was performed;the flow rate was 1.0 ml/min;the detection wavelength was 210 nm and 325 nm,the column temperature was 30℃,the injection volume was 10μl.Results Within the following ranges,the linear relationship of eight components were good respectively:7.61～304.40 μg/ml for fumaric acid(R2 = 0.999 7),3.52～140.60 μg/ml for neochlorogenic acid(R2 = 1.000 0),4.99～199.60 μg/ml for chlorogenic acid(R2 = 1.000 0),3.89～155.40 μg/ml for cryptochlorogenic acid(R2 = 1.000 0),0.56～22.06 μg/ml for caffeic acid(R2 = 0.999 9),0.57～22.62 μg/ml for isofraxidin(R2 = 1.000 0),26.75～1 070.00 μg/ml for astilbin(R2 = 1.000 0),12.49～499.40 μg/ml(R2 = 0.999 9)for rosmarinic acid(R2 = 1.000 0),the recovery rate of different addition levels was 95.05%～99.26%,and its relative standard deviation was between 1.15%and 1.93%.Conclusion In the stem,the content of fumaric acid,chlorogenic acid,caffeic acid,astilbin,rosmarinic acid were 1 year＞2 years＞3 years;the content of neochlorogenic acid,cryptochlorogenic acid were 1 year＜2 years＜3 years;the content of isofraxidin was 1 year＞3 years＞2 years.In the leaves,the content of fumaric acid,neochlorogenic acid,cryptochlorogenic acid,caffeic acid,isofraxidin,astilbin,rosmarinic acid were 1 year＞2 year＞3 years;the content of chlorogenic acid was 1 year＞3 years＞2 years.
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