银杏叶提取物对脓毒症相关性脑病认知功能及海马炎症因子的影响
The effect of Ginkgo biloba extract on cognitive function and hippocampal inflammatory factors in sepsis associated encephalopathy
王旭 1曲艳平 1贺文廷 1董浩 1范智东2
作者信息
- 1. 大理大学临床医学院,云南大理 671000
- 2. 大理大学第一附属医院麻醉科,云南大理 671000
- 折叠
摘要
目的 探究银杏叶提取物(GBE)对脓毒症相关性脑病(SAE)小鼠认知功能及海马炎症因子的影响.方法 使用随机数字表法将C57BL/6J小鼠分为假手术组(Sham组)、模型组(SAE组)、银杏叶提取物组(GBE+SAE组)、头孢曲松钠组(CS+SAE组)4 组,每组12 只,并通过盲肠结扎穿孔(CLP)构建SAE模型.各组小鼠进行旷场实验、新物品识别实验和Y迷宫自发交替实验,行为学实验结束后取材;免疫组化法检测海马Bax和Bcl-2 表达情况;ELISA法测定海马肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)表达水平.结果 SAE组小鼠认知功能受损,海马Bax表达增加,Bcl-2 表达下降,TNF-α和IL-6 含量均升高;给予GBE干预后可下调海马Bax和上调Bcl-2 表达,降低海马TNF-α和IL-6 含量.结论 GBE可改善SAE小鼠认知功能障碍,这可能与其抑制海马神经炎症、阻止神经元凋亡有关.
Abstract
Objective Exploring the effects of Ginkgo biloba extract(GBE)on cognitive function and hippocampal inflammatory factors in sepsis associated encephalopathy(SAE)mice.Methods C57BL/6J mice were randomly divided into four groups included sham surgery group(Sham group),SAE model group(SAE group),Ginkgo biloba extract group.(GBE+SAE group),and ceftriaxone sodium group(CS+SAE group)by using random number table method,with 12 mice in each group.SAE models were established through cecal ligation and puncture(CLP).Behavioral tests including open field test,novel object recognition test,and Y-maze spontaneous alternation test were conducted on mice in each group,followed by sample collection.Immunohistochemistry was performed to assess the expression of Bax and Bcl-2 in the hippocampus,while ELISA was employed to measure the levels of TNF-α and IL-6 expression.Results SAE group mice showed cognitive impairment with increased hippocampal Bax and decreased Bcl-2 expression,alongside elevated TNF-α and IL-6 levels.GBE intervention downregulated hippocampal Bax,upregulated Bcl-2 expression,and reduced TNF-α and IL-6 levels.Conclusion GBE improves cognitive dysfunction in SAE mice,likely by inhibiting neuroinflammation and neuronal apoptosis in the hippocampus.
关键词
银杏叶提取物/脓毒症相关性脑病/认知功能Key words
Ginkgo biloba extract/Sepsis associated encephalopathy/Cognitive function引用本文复制引用
基金项目
云南省教育厅科研项目(2023Y0966)
出版年
2024
NETL
NSTL
万方数据