Quality evaluation of Citri Reticulate Pericarpium and Citri Reticulate Pericarpium Viride based on fingerprint and multi index component content determination
Objective The fingerprint spectra and multi index component content determination of Citri Reticulate Pericarpium(CRP)and Citri Reticulate Pericarpium Viride(CRPV)were established,combined with fingerprint similarity evaluation,cluster analysis(CA),and partial least squares discriminant analysis(PLS-DA)to evaluate the quality of multiple batches of CRP and CRPV from different origins.Methods Using Agilent ZORBAX SB C18 chromatography column(250 mm×4.6 mm,5 μm)acetonitrile 0.1%phosphoric acid water as the mobile phase for gradient elution,with a flow rate of 1.0 ml/min,column temperature of 30℃,and detection wavelength of 330 nm.Establish fingerprint spectra for 10 batches of CPR and 24 batches of CRPV from different regions,identify common peaks,and perform similarity analysis.Results Simultaneously determine the contents of naringin,hesperidin,coumarin,quercetin and hesperidin.The similarity of HPLC fingerprint spectra for 10 batches of CRP and 24 batches of CRPV was above 0.9.11 common peaks were identified for CRP and 13 common peaks were identified for CRPV.A total of 7 peaks were identified,including narirutin,hesperidin,neohesperidin,neoponcrin,nobiletin,tangeritin and 5-demethynobiletin.10 batches of CRP and 24 batches of CRPV[12 batches of Fructus Citri Immaturus(FCI)and 12 batches of Pericarpium Citri Reticulatae Viride(PCRV)]can be divided into 2 categories through CA and PLS-DA.Through VIP analysis under PLS-DA,the common components of CRP and CRPV(narirutin,hesperidin,neoponcrin,nobiletin,tangeritin content are 4.6～10.82,44.77～131.31,1.31～4.34,0.33～5.72,0.08～3.54 mg/g,respec-tively)were screened,and hesperidin,neoponcrin and tangeritin were be regarded as chemotaxonomic markers of the common quality differences between CRP and CRPV.Conclusion The quality evaluation method for CRP and CRPV established in this study is simple,sensitive,accurate,and has good repeatability,which can provide a basis for the quality evaluation of CRP and CRPV.
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