Determination of gallic acid and ellagic acid in Castanopsis sclerophylla ultrafine powder by HPLC
Objective Establish a method for the determination of gallic acid and gallogen in Castanopsis sclerophylla ultrafine powder with high ac-curacy.Methods The effective components in Castanopsis sclerophylla were extracted by ultrafine powder technique and was detected on a high performance liquid chromatograph using a reversed-phase column.The gallic acid and gallogen contents were quantified at 270 nm using a mobile phase of acetonitrile and 0.1%phosphoric acid aqueous solution with gradient elution.Results Gallic acid in the concentration range of 0.415 8～10.395 0 μg/ml(r2=0.998 1)and gallogen in the concentration range of 0.180 1～4.502 0 μg/ml(r2=0.999 8)both had a good linearity with the peak area.The average recoverie of gallic acid was 97.52%,RSD of 6 samples was 2.10%.The average recoverie of gallogen was 98.36%,and RSD of 6 samples was 1.77%.Conclusion The method which can be used for the quality control of Castanopsis sclerophylla ultrafine powder is simple,accurate and reproducible.
NETL
NSTL
万方数据
