苦槠超微粉中没食子酸、鞣花酸含量测定方法的建立
Determination of gallic acid and ellagic acid in Castanopsis sclerophylla ultrafine powder by HPLC
韦天宝 1吴凯伦 2龚雪媛 2沈子博 2赵静芳 2周玲娜 2蒋士鹏2
作者信息
- 1. 广西维威制药有限公司,南宁 530031
- 2. 金华市食品药品检验检测研究院,浙江金华 321000
- 折叠
摘要
目的 建立检测苦槠超微粉中没食子酸、鞣花酸含量准确度较高的方法.方法 采用超微打粉技术提取苦槠果实中的有效成分,并使用反相色谱柱在高效液相色谱仪上检测含量.选用乙腈以及 0.1%磷酸水溶液作为梯度洗脱流动相,在 270 nm波长下测定没食子酸和鞣花酸含量.结果 没食子酸和鞣花酸分别在 0.415 8～10.395 0 μg/ml(r2=0.998 1)、0.180 1～4.502 0 μg/ml(r2=0.999 8)的浓度范围内与峰面积有较好的线性关系,平均加样回收率:没食子酸为 97.52%(6 份样品RSD为 2.10%),鞣花酸为 98.36%(6 份样品RSD为1.77%).结论 本方法操作简便、准确度及重复性良好,可为苦槠超微粉的质量研究提供依据.
Abstract
Objective Establish a method for the determination of gallic acid and gallogen in Castanopsis sclerophylla ultrafine powder with high ac-curacy.Methods The effective components in Castanopsis sclerophylla were extracted by ultrafine powder technique and was detected on a high performance liquid chromatograph using a reversed-phase column.The gallic acid and gallogen contents were quantified at 270 nm using a mobile phase of acetonitrile and 0.1%phosphoric acid aqueous solution with gradient elution.Results Gallic acid in the concentration range of 0.415 8～10.395 0 μg/ml(r2=0.998 1)and gallogen in the concentration range of 0.180 1～4.502 0 μg/ml(r2=0.999 8)both had a good linearity with the peak area.The average recoverie of gallic acid was 97.52%,RSD of 6 samples was 2.10%.The average recoverie of gallogen was 98.36%,and RSD of 6 samples was 1.77%.Conclusion The method which can be used for the quality control of Castanopsis sclerophylla ultrafine powder is simple,accurate and reproducible.
关键词
高效液相色谱法/苦槠超微粉/没食子酸/鞣花酸/含量Key words
HPLC/Castanopsis sclerophylla ultrafine powder/Gallic acid/Gallogen/Content引用本文复制引用
出版年
2024
NETL
NSTL
万方数据