不同牛血清替代人血清作为空白基质在HPLC-MS/MS法测定他克莫司药物浓度中的可行性分析
Study on the feasibility analysis of different bovine serum replacing human serum as blank substrate for determination of tacrolimus drug concentration by HPLC-MS/MS method
朱双燕 1孔艳 2杜秋莹 1邹进 1李燕 1蒲强红 1刘易陇1
作者信息
- 1. 乐山市人民医院药学部,四川乐山 614000
- 2. 南充市中医医院药学部,四川南充 637000
- 折叠
摘要
目的 以他克莫司为模型药,考察不同牛血清替代人血清作为空白基质在HPLC-MS/MS药物浓度测定中的可行性研究.方法 在相同的处理方法下,以人血清和不同牛血清作为空白基质制备标准曲线,对比 101 份他克莫司血清样品在不同标准曲线下的浓度是否存在差异.结果 三种牛血清(小牛血清、新生牛血清、胎牛血清)与人血清的相对偏差均大于TEa(12.5%),均不能替代人血清作为空白基质.结论 以他克莫司为模型药时,在HPLC-MS/MS法测定药物浓度中,三种牛血清均不能替代人血清作为空白基质.
Abstract
Objective Using tacrolimus as the model drug,the feasibility analysis of different bovine serum replacing human serum as blank substrate was investigated in the determination of drug concentration by HPLC-MS/MS.Methods Under the same treatment method,human serum and different bovine serum were used as blank matrix to prepare standard curves,and the concentration of 101 tacrolimus serum samples was com-pared under different standard curves.Results The relative deviation between the three types of bovine serum(calf serum,newborn bovine serum,fetal bovine serum)and human serum was greater than TEa(12.5%),and they could not replace human serum as blank substrate.Conclusion When tacrolimus was used as the model drug,none of the three bovine serum could replace human serum as the blank substrate in the determination of drug concentration by HPLC-MS/MS.
关键词
高效液相色谱-串联质谱/药物浓度监测/空白基质/他克莫司Key words
HPLC-MS/MS/TDM/Blank matrix/Tacrolimus引用本文复制引用
基金项目
乐山市重点科技计划项目(22SZD075)
出版年
2024
NETL
NSTL
万方数据