Fluorescence quantitative nucleic acid amplification technique and ring-mediated isothermal amplification technique in respiratory tract comparative study in the detection of infectious pathogens
Objective To compare the application value of fluorescence quantitative nucleic acid amplification and ring-mediated isothermal am-plification in the detection of respiratory tract infection pathogens.Methods A total of 120 patients with respiratory tract infection were randomly selected from Hedong District Center for Disease Control and Prevention of Tianjin City from January 2020 to January 2023.Fluorescent quantitative nucleic acid amplification(PCR)and loop-mediated isothermal amplification(LAMP)were used to detect respiratory tract pathogens,and the detection results were compared and analyzed.Results Bacterial infection was detected by LAMP in 85 of 120 patients(positive rate was 70.83%).Bacterial infection was detected by fluorescence quantitative PCR in 72 cases(positive rate 60.00%),the difference was not statistically significant(P＞0.05).Among them,the detection rate of methicillin-resistant Staphylococcus,Streptococcus pneumoniae and Haemophilus influenzae by LAMP method was higher than that by fluorescence quantitative PCR method,and the difference was statistically significant(P＜0.05).Fluorescent quantitative PCR and LAMP method were used to detect 10 pathogens of respiratory tract infections.The results showed that the detection of methicillin-resistant Staphylococcus and Haemophilus influenzae by LAMP method had excellent consistency.The detection of methicillin-resistant Staphylococcus by fluorescence quantitative PCR had excellent cousistency(Kappa≥0.75).Other pathogens were detected by fluorescent quantitative PCR and LAMP with moderate consistency(0.4≤Kappa＜0.75).Conclusion Both fluorescent quantitative PCR and LAMP methods have higher detection rates for respiratory infection pathogens,and the detection rate of LAMP method for methicillin-resistant Staphylococcus,Streptococcus pneumoniae and Haemophilus influenzae is higher than that of fluorescent quantitative PCR method.
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