Drug virtual screening of LSM7 inhibitors and verification of their role in promoting apoptosis of breast cancer cells
Objective To conduct drug virtual screening for LSM7 inhibitors and verify whether LSM7 inhibitors can promote apoptosis of breast cancer cells.Methods Gene expression data of breast cancer were downloaded and analyzed from public databases.Through bioinformatics analysis,study the expression of LSM7 in breast cancer.Use LSM7 as the target for drug virtual screening of Valrubicin as an LSM7 inhibitor.Determine the concentration gradient of Valrubicin using the MTT method.Treat cells with different concentrations of Valrubicin and detect the pro-apoptotic effect of Valrubicin on breast cancer MCF-7 cells using flow cytometry analysis.Validate the effect of Valrubicin on LSM7 expression and the expression of apoptosis-related proteins P53,Bcl-2,and Bax using western blot analysis.Results LSM7 was significantly overexpressed in breast cancer.Drug virtual screening results identify Valrubicin as a potential optimal drug for LSM7 inhibition.MTT results show concentration-dependent and time-dependent inhibition of MCF-7 cell proliferation by Valrubicin.Flow cytometry analysis demonstrates a significant increase in early and late apoptosis rates in MCF-7 cells after Valrubicin treatment.Western blot analysis confirms that Valrubicin promotes apoptosis of breast cancer cells by downregulating LSM7,Bcl-2 expression,upregulating P53,Bax expression.Conclusion LSM7 is highly expressed in breast cancer,and Valrubicin as an LSM7 inhibitor,promotes apoptosis in breast cancer cells.
万方数据
维普
