Establishment of the organoids for ovarian cancer and its application in drug sensitivity test
Objective To successfully construct ovarian cancer organoid models by using clinical samples,select the commonly used first-line drug carboplatin in ovarian cancer clinical practice,explore the impact of different treatment factors on the drug sensitivity test results of ovarian cancer organoid,and provide a basis for personalized medication and precise treatment plan selection for tumor patients.Methods Two cases of ovarian cancer organoids OC-1 and OC-2 were treated with different cell densities,drug doses,and drug action times.Control and treatment groups were set up to compare and analyze the sensitivity of the two cases of ovarian cancer organoids to carboplatin.In terms of drug doses,six different concentrations of carboplatin(drug doses of 100,50,10,1,0.1,0.01 μmol/L)were designed to apply in the two cases of ovarian cancer organoids.In terms of drug action time,at the same dose of 100 μmol/L carboplatin acted on two cases of ovarian cancer organoids for 3～9 days.In the cell drug sensitivity test,CCK-8 method was used to detect cell proliferation activity,and the impact of different treatment factors on the drug sensitivity test results of ovarian cancer organoids was compared and analyzed based on the relative inhibition rate(T/C)of tumor cells.Results ①In the selection of cell density,when inoculating 8 000 and 4 000 cells/well,50～100 μmol/L carboplatin had a significant inhibitory effect on the growth of ovarian cancer organoids OC-1 and OC-2(P＜0.05),and there was no statistically significant difference in their inhibitory effects(P＞0.05).②In the selection of different drug doses,50～100 μmol/L carboplatin was effective in vitro for ovarian cancer organoids OC-1 and OC-2(T/C≤50%),while the other fore dose groups were ineffective in vitro.③In the selection of drug action time,100 μmol/L carboplatin showed a more significant growth inhibitory effect on organoids OC-1 and OC-2 over a 3～9 day observation period(P＜0.01 or P＜0.001).Conclusion By simulating the real tumor environment in vivo through ovarian cancer organoids,the chemotherapy drug carboplatin affects the drug sensitivity test results of ovarian cancer drugs at different doses and action times.The use of ovarian cancer organoids to design personalized medication plans for ovarian cancer patients has a very positive significance.
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