Determination of adonifrinine content of Senecio scandens Buch.-Ham.formula granules based on standard decoction
Objective The content of adonifrinine in Senecio scandens Buch.-Ham.decoction pieces and standard decoction was determined,and the content of adonifrinine in Senecio scandens Buch.-Ham.decoction pieces and standard decoction was analyzed based on the transfer rate,which provided a reference for the quality evaluation of Senecio scandens Buch.-Ham.formula granules.Methods Ultra-performance liquid chromatography-mass spectrometry(UPLC-MS/MS)was used for detection.The column was C18(HypersiL GOLD,100 mm×2.1 mm,1.9 μm),and the mobile phase was acetonitrile-0.5%formic acid(7∶93),column temperature 30℃,flow rate 0.3 ml/min,injection volume 2 μl.A triple quadrupole mass detector was used for detection in electrospray ionization(ESI)positive ion mode,with ion monitoring channels m/z of 366(adonifroline)and 326(monocronitine).Results The content of adonifrinine in 15 batches of Senecio scandens Buch.-Ham.decoction pieces ranged from 0～31.6 μg/g,with an average value of 16.7 μg/g.The ointment yield of standard decoction ranged from 10.2%～21.6%,and the average value was 16.1%.Adonifroline was not detected in 5 of the 15 batches of decoction pieces,and the content of the remaining 10 batches of standard decoction adonifrinine ranged from 48.7 to 138.5 μg/g,with an average value of 109.3 μg/g and the±30%range of 76.5～142.1 μg/g.The base transfer rate of adonifrinine ranged from 40.4%to 97.3%,with a mean of 70.0%.Conclusion Senecio scandens Buch.-Ham.is a commonly used Chinese herbal medicine,and adonifrinine is a toxic component of Senecio scandens Buch.-Ham.,this study provides a certain reference for the study of Senecio scandens Buch.-Ham.formula granules,and is of great significance for the content limit control of Senecio scandens Buch.-Ham.formula granules.
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