Study on quality improvement of Xiaoshi Jianwei Pian based on HPLC and TLC
Objective To improve the quality standard of Xiaoshi Jianwei Pian through scientific analytical methods.Methods Malt and betel nut in the Xiaoshi Jianwei Pian were identified by thin layer chromatography(TLC);the main contents of ursolic acid and onolic acid in the hawthorn extract were precisly determined by high-performance liquid chromatography(HPLC).Experimental conditions included:DiKMAC18 column(4.6 mm×250 mm,5 μ m),mobile phase of acetonitrile-methanol-0.5%ammonium acetate solution(67∶12∶21),flow rate of 1.0 ml/min,column temperature of 30℃,and the detection wavelength of 210 nm.Results TLC results showed that the spots of malt and betel nut were clear,with no interference from the negative control solution.HPLC results indicated that the content determination of ursolic acid at 36.843 12～368.431 2 μg/ml had a linear regression equation of Y=4.587 4 X+24.042,with a correlation coefficient R2=0.999 5 and an average recovery rate of 99.23%.The content determination of onolic acid at 10.36 to 103.6 μg/ml had a linear regression equation of Y=5.789 7 X+8.565 8,with a correlation coefficient R2=0.999 2 and an average recovery of 98.99%.Conclusion The established method in this study has high accuracy and strong reliability,which can provide a basis for improving the quality standard of Xiaoshi Jianwei Pian.
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