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HPLC梯度洗脱法测定雷公藤多苷片中甲素的含量

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目的 建立以梯度洗脱测定市售雷公藤多苷片中甲素含量的方法.方法 采用HPLC以甲醇-水为流动相梯度洗脱;流速为 0.9 ml/min;柱温为 25℃;检测波长为 218 nm.结果 线性范围为 0.65~41.68 μg/ml,r=0.999 8,平均回收率 103.43%,RSD=0.96%(n=9).结论 梯度洗脱法比现行质量标准的检测方法重复性更好、精密度更高.可作为雷公藤制剂中甲素的定量检测方法.
Using HPLC with gradient elution to detect triptolide content in Tripterygium glycosides tablets
Objective To establish a method to determine the content of triptolide in Tripterygium glycosides tablets by HPLC with gradient elution.Methods The high performance liquid chromatography external standard method was adopted with methanol aqueous solution with gradient elution at a flow rate of 0.9 ml/min,column temperature of 25℃and detection wavelength of 218 nm.Results The linear relationship was good(r=0.999 8)in the range of 0.65 to 41.68 μg/ml with the average recovery of 103.43%and the RSD of 0.96%(n=9).Conclusion Compared to the detection method of triptolide content in the drug standard,the gradient elution is better in reproducibility and higher in accuracy.It can be used as a quantitative method for the quantitative analysis of triptolide in Tripterygium glycosides tablets.

Tripterygium glycosides tabletsTriptolideGradient elutionHPLC

陈静、徐荣霞、林健

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江苏美通制药有限公司,江苏泰州 225300

雷公藤多苷片 雷公藤甲素 梯度洗脱 HPLC

2024

中国处方药
南方医药经济研究所

中国处方药

影响因子:0.649
ISSN:1671-945X
年,卷(期):2024.22(9)
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