中国处方药2024，Vol.22Issue(10) ：53-55.

现代分析技术在破伤风类毒素的结构表征中的应用

Application of modern analytical techniques in structural characterization of tetanus toxoid

杨海艳 刘贤雪 杜剑晖 罗玲 詹益雄 车腾 郭长福 瞿明霞
中国处方药2024，Vol.22Issue(10) ：53-55.
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现代分析技术在破伤风类毒素的结构表征中的应用

Application of modern analytical techniques in structural characterization of tetanus toxoid

杨海艳 1刘贤雪 1杜剑晖 1罗玲 1詹益雄 1车腾 1郭长福 1瞿明霞1
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作者信息

  • 1. 武汉生物制品研究所有限责任公司,武汉 430207
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摘要

目的 采用现代分析技术对破伤风类毒素(TT)的结构进行表征.方法 采用圆二色谱法(CD)对TT的二级结构进行考察,差示扫描量热法(DSC)对TT的变性温度进行考察,并对不同批次样品的测定结果进行比较.结果 9 批次TT的α-螺旋比例为25.4%～26.2%,反向平行为 12.3%～14.9%,平行为 6.4%～7.0%,β-转角为 21.7%～24.0%,无规卷曲为 31.8%～33.9%,熔解温度为89.19℃～90.47℃,结果重复性、批间一致性均良好.结论 圆二色谱法和差示扫描量热法可用于破伤风类毒素的结构表征,为该类产品在生产过程中的稳定性和一致性提供技术支持.

Abstract

Objective To characterize the structure of tetanus toxoid(TT)using modern analytical techniques.Methods Circular dichroism(CD)was used to investigate the secondary structure of TT,differential scanning calorimetry(DSC)was used to investigate the denaturation temperature of TT,then the determination results of different batches of samples were compared.Results 9 batches of TT α-helix ratio is 25.4%～26.2%.The anti-parallel is 12.3%～14.9%.The parallel is 6.4%～7.0%.The β-turn is 21.7%～24.0%,the random coil is 31.8%～33.9%,and the melting temperature is 89.19℃～90.47℃.The repeatability and inter batch consistency of the results are good.Conclusion Circular dichroism and differential scanning calorimetry can be used for structural characterization of tetanus toxoids,providing technical support for the stability and consistency of such products in the production process.

关键词

破伤风类毒素/圆二色谱/差示扫描量热法/二级结构/变性温度

Key words

Tetanus toxoid/Circular dichroism/Differential scanning calorimetry/Secondary structure/Denaturation temperature

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出版年

2024
中国处方药
南方医药经济研究所

中国处方药

影响因子：0.649
ISSN：1671-945X
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