Mechanisms study of attenuation of compatibility of Tripterygium wilfordii Hook.f.and licorice based on network pharmacology and UPLC-Q-TOF-MS
Objective To analyze the chemical constituents in Tripterygium wilfordii Hook.f.single decoction and Tripterygium wilfordii Hook.f.-licorice mixed decoction based on UPLC-Q-TOF-MS technology,aiming to elucidate the mechanism behind licorice's role in reducing the liver toxicity of Tripterygium wilfordii Hook.f.through network pharmacology.Methods Liquid chromatography-mass spectrometry was utilized to analyze the chemical constituents in Tripterygium wilfordii Hook.f.single decoctions and Tripterygium wilfordii Hook.f.-licorice mixed decoction,assessing the variations in chemical profiles before and after the combination.Utilizing the component analysis results,the chemical constituents,constituents related targets,and liver injury-related targets were identified and analyzed via databases like TCMSP,DAVID,and DisGeNet,and a Venn diagram was constructed,and the common targets were entered into the String database to build a PPI network,which was visualized by using Cytoscape software,and the core therapeutic targets were screened for GO and KEGG enrichment analyses.Results Compared to Tripterygium wilfordii Hook.f.single decoction,there were 27 components reduced in the Tripterygium wilfordii Hook.f.-licorice mixed decoction,which was identified by the UPLC-Q-TOF-MS technology.And 146 common targets were obtained from the components of Tripterygium wilfordii Hook.f.single decoction and liver injury,244 common targets were obtained from the components of Tripterygium wilfordii Hook.f.-licorice mixed decoction and liver injury.PPI network analysis identified targets such as TNF,IL-6,and SRC as potential key targets for liver injury caused by Tripterygium wilfordii Hook.f.,Akt1 and TP53 as key targets for mitigating liver injury through the combination of Tripterygium wilfordii Hook.f.and licorice.KEGG analysis demonstrated that the biological processes of the AGE-RAGE signaling pathway,lipid and atherosclerosis,PI3K-Akt signaling pathway,MAPK signaling pathway,and TNF signaling pathway likely constitute the effective pathways for mitigating liver injury through the combination of Tripterygium wilfordii Hook.f.and licorice.Conclusion The combination of Tripterygium wilfordii Hook.f.and licorice exerts effect to reduce liver toxicity through multi-target,multi-pathway.
万方数据
维普
