Analysis of the action mechanism of basic traditional Chinese medicine combinations for the treatment of insomnia based on network pharmacology
Objective To explore the molecular mechanism of the combination of traditional Chinese medicine basic drugs for treating insomnia based on network pharmacology.Methods Retrieve clinical randomized controlled trial literatures on the combined treatment of Chinese and Western medicine for insomnia from databases such as CNKI,Wanfang,and PubMed from the establishment of the database to January 2023,and count all Chinese herbal medicines included in the literatures for insomnia treatment.Use IBM SPSS Modeler 18.0 software for association rule analysis,and select drug combinations with high support rate and confidence as the basic Chinese herbal formula.Query the main chemical components and targets of various traditional Chinese medicines in combination drugs through the TCMSP database.Screen using keywords such as"insomnia"and"sleep disorder"in DrugBank,GeneCards,OMIM,DisGenet,and TTD databases to obtain insomnia related targets.Use the String platform for protein-protein interaction analysis,construct a PPI network,and explore potential protein functional modules in the network.KEGG signaling pathway analysis and GO functional enrichment analysis were performed on all key targets using the Metascape platform.Then,a"Combination Drug Components-Insomnia-Pathway"network was constructed using Cytoscape 3.8.2 software.Finally,the core target proteins and active ingredients of the combination drug in treating insomnia were validated through molecular docking using the RCSB PDB database,AutoDock to verify the binding activity between the active ingredients and the target.Results After literature search and screening,a total of 90 articles were obtained.Based on association rules analysis of the combination of Chinese medicines for treating insomnia,basic Chinese medicine combinations with high support rate and confidence were selected(Licorice,White peony,Sour jujube kernel,Yuanzhi).The core active components of the combined therapy for insomnia include Quercetin,Kaempferol,7-methoxy-2-methylisoflavone,and Isovioline.The core targets include IL-6,AKT1,TNF,IL-1β,etc.Molecular docking results also show that 83.93%of the binding energy values were less than-5 kcal/mol,indicating that most of the targets have good binding activity with the components.The biological pathway of combined medicine therapy for insomnia mainly acts on pathways such as lipid and atherosclerosis,neuroactive ligand-receptor interaction,tumor pathways,and chemical carcinogenesis-receptor activation.Conclusion This study preliminarily reveals the mechanism of action of multi-component-multi target-multi pathway mechanism of traditional Chinese medicine combination therapy for insomnia,providing a basis for the clinical development and utilization of insomnia drugs.
万方数据
维普
