Objective To investigate the application effect of disposable straight intravenous catheter in the C-peptide test of diabetic patients.Methods A total of 316 inpatients with diabetes admitted to Nanjing Jiangbei Hospital from January 2021 to December 2022 were selected as the study objects,151 patients admitted from January to December 2021 received routine blood collection(control group),and 165 patients admitted from January to December 2022 received blood collection using a disposable straight intravenous catheter(observation group).The results of the C-peptide test and blood collection were compared between the two groups.Results There was no significant difference between the two groups and in fasting,at 30,60,120 and 180 min after taking sugar or steamed bread(P>0.05).The sealed blood collection time,puncture times and numerical rating scale(NRS)score of the observation group were lower than those of the control group,and the blood collection satisfaction of the observation group was higher than that of the control group,with statistical significance(P<0.01).Conclusion Disposable straight intravenous catheter will not cause a negative impact on the results of C-peptide of diabetic patients,and help to reduce the operation time and puncture times of blood collection,and improve patient's satisfaction.