Cloning and Tissue Expression of FGF1 Gene from Tibetan Sangsang Yak
In order to study of the biological structure and function of the FGF1 gene in yak and its expression patterns in different tissues,the CDS region sequence of the FGF1 gene was cloned using RT-PCR technique with cDNA from the heart tissue of Sangsang yak as a template.Bioinformatics analysis was performed on the cloned sequence.The ex-pression levels of the FGF1 gene in the heart,liver,spleen,lung,renal,muscle,and fat tissues of the yak were detected using real-time fluorescence quantitative PCR(qRT-PCR).The results showed that the CDS region sequence of the FGF1 gene in Sangsang yak was 468 bp,encoding a total of 155 amino acids.Similarity alignment revealed that Sang-sang yak had the highest homology with Bos taurus and Bos indicus(99.8%),and the lowest homology with Gallus gal-lus(73.7%).Bioinformatics analysis indicated that the theoretical isoelectric point of the FGF1 protein in Sangsang yak was 6.51,indicating that it is an acidic and hydrophilic protein.It had no signal peptide and transmembrane structure,and was predominantly located in the cell nucleus.The protein possessed 7 glycosylation sites and 29 potential phos-phorylation sites.The secondary structure of the protein was mainly composed of random coil structures and exhibited significant interactions with proteins such as FGF22,TGFB1 and FGF17.The results of real-time fluorescence quanti-tative analysis showed that the FGF1 gene was expressed in various tissues of Sangsang yak to different degrees,with the highest expression level in the lung tissue,significantly higher than in other tissues(P<0.01).
国家科技期刊平台
NSTL
万方数据
维普
