摘要
为探讨可能影响动物毛色的10个候选基因(TCF25、TYR、TYRP1、ASIP、KIT、MITF、EDNRB、SLC7A11、MC1R和DCT)与巴什拜羊毛色的关系,利用实时荧光定量PCR技术对30只1周岁不同毛色(棕色、白色和黑色各10只)被毛的巴什拜羊皮肤组织中与毛色相关基因的mRNA表达量进行了研究,所有数据使用SPSS 23.0进行单因素方差分析.结果显示,在不同毛色被毛巴什拜羊皮肤中,TCF25、ASIP和EDNRB基因在白色被毛皮肤中的表达量均显著高于黑色和棕色被毛皮肤(P<0.05);TYR、TYRP1、DCT和MC1R基因在黑色被毛皮肤中的表达量均显著高于白色和棕色被毛皮肤(P<0.05);MITF基因在棕色被毛皮肤中的表达量显著高于白色和黑色被毛皮肤(P<0.05),而KIT和SLC7A11基因在不同毛色皮肤组织中均无显著差异(P>0.05).综上可知,TCF25和ASIP基因主要与巴什拜羊白色被毛性状相关,TYR、TYRP1、DCT和MC1R主要与巴什拜羊黑色被毛性状相关,而MITF和EDNRB基因参与调控巴什拜羊棕色和白色被毛性状的具体作用机制还有待进一步探究.
Abstract
In order to explore the relationship between ten candidate genes(TCF25,TYR,TYRP1,ASIP,KIT,MITF,EDNRB,SLC7A11,MC1R and DCT)that may affect animal coat color and Bashibai sheep coat color,qRT-PCR was used to study the mRNA expression of coat color related genes in the skin of 30 Bashibai sheep at the same year of age with different coat colors(10 black,10 white and 10 brown coat).All data were analyzed by SPSS 23.0 for one-way ANOVA.The results showed that the expression of TCF25,ASIP and EDNRB genes in skin of white coat Bashibai sheep was significantly higher than that in skin of black and brown coat(P<0.05),and the expression of TYR,TYRP1,DCT and MC1R genes in skin of black coat was significantly higher than that in skin of white and brown coat(P<0.05).The expression of MITF gene in skin of brown hair was significantly higher than that in skin of white and black(P<0.05),but there was no significant difference in KIT and SLC7A11 genes in different coat color skin(P>0.05).In summary,TCF25 and ASIP genes mainly related the white coat color traits of Bashbai sheep,and TYR,TYRP1,DCT and MC1R mainly related the black wool color traits of Bashbai sheep,while the specific mechanisms of MITF and EDNRB genes involved in regulating the brown and white coat color traits of Bashbai sheep need to be further explored.
基金项目
新疆维吾尔自治区自然科学基金(2021D01B32)
国家科技期刊平台
NSTL
维普
万方数据