西门塔尔牛输精后21天彩色多普勒预判妊娠方法的建立
Establishment of Method to Predict Pregnancy by Color Doppler at 21 Days After Insemination in Simmental Cattle
艾力亚木·土尔衮 1常站站 1古力孜巴·艾力 1李伟建 1梁亦白 1王莉 1刘娅兰 1王旭光1
作者信息
- 1. 新疆农业大学动物科学学院,乌鲁木齐 830052
- 折叠
摘要
为建立应用彩色多普勒开展黄体血流评估、预判西门塔尔牛在配后21d妊娠情况的方法,选择98头西门塔尔牛进行定时输精,配后21d使用B超、彩色多普勒进行卵巢检查,采集黄体的最大面积、黄体血流面积;同时采集血样分析激素水平,在配后35d进行B超孕检,对比分析妊娠情况.结果表明,在配后21d通过主观预判检出8头空怀牛,以血流面积为预判标准预判检出26头空怀牛,以血流黄体比预判标准预判检出23头空怀牛,配后35dB超检查出36头空怀牛;妊娠牛孕酮(P4)、雌二醇比孕酮(E2/P4)水平均极显著高于空怀牛(P<0.01);使用血流黄体比预判标准对空怀牛的灵敏性为100%,可以用该方法尽早发现空怀牛,相较于配后30～35dB超检查妊娠,配后21d彩色多普勒检查可以让空怀牛提前9～15 d再次进行定时输精.
Abstract
The purpose of this paper was to establish a method for using color Doppler to evaluate luteal blood flow and predict pregnancy status of Simental cow at 21 days after insemination.98 Simental cows were selected for timed artificial insemination(AI).The ovaries were examined by B type ultrasound and color Doppler at 21 days after insemi-nation,and the maximum area of the corpus luteum and the area of corpus luteumblood flow were collected;at the same time,blood samples were collected to analyze the hormone level,and ultrasound pregnancy determination was performed at 35 days after insemination.The results showed that 8 unpregnant cows were detected by subjective pre-judgement at 21 days after AI,26 unpregnant cows were checked out under the blood flow area as a criterion,23 un-pregnant cows were detected with blood flow luteal ratio prejudgement,and 36 unpregnant cows were detected by ultra-sound examination at 35 days after AI;the levels of progesterone,and ratio of estradiol to progesterone in pregnant cows were significant higher than those in unpregnant cows(P<0.01);the prognostic criterion of the blood flow area luteal area ratio was 100%sensitive for unpregnant cows,nulliparous cows can be detected as early as possible by this method,and compared with ultrasound examination of pregnancy 30-35 days after AI,color Doppler examination 21 days after AI allows unpregnant cows to undergo timed artificial insemination again 9-15 days earlier.
关键词
西门塔尔牛/早孕检查/彩色多普勒/曲线下面积/空怀预判Key words
Simental cattle/early pregnancy detection/color Doppler/area under the curve/empty pregnancy prediction引用本文复制引用
基金项目
自治区现代畜禽种业提升专项(2024)(2024XM-22)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据