Cloning and Bioinformatics Analysis of MYL7 Gene in Sangsang Yak
The CDS region of Myosin light chain 7(MYL7)gene of Tibetan Sangsang yak was cloned,and bioinforma-tics analysis was carried out to detect the expression difference of this gene in various tissues,so as to provide a theo-retical basis for exploring the function of this gene.The CDS region sequence was cloned by PCR using the cDNA of Sangsang yak muscle tissue as a template,and bioinformatics analysis was performed using a variety of online biologi-cal software and tools.The results showed that the CDS region of MYL7 gene was 447 bp in length and encoded 148 amino acids.The molecular formula of the protein encoded by this gene was C743H1155N187O237S7,the number of atoms was 2 329,the theoretical isoelectric point was 4.37,the instability coefficient and the total average hydrophilicity were 36.02 and-0.364,respectively,which were stable hydrophilic proteins.There are 11 specific phosphorylation sites,in-cluding 5 serine,5 threonine and 1 tyrosine.MYL7 protein has no signal peptide and transmembrane helix structure,which belongs to non-secretory protein.Through subcellular localization,it was found that the MYL7 gene of Sangsang yak was distributed in mitochondria,nucleus,cytoplasm and cell membrane.The advanced structure of the protein was mainly α-helix and random coil.By constructing a phylogenetic tree,it was found that Sangsang yak and wild yak had the closest relationship,which was in line with the actual situation,indicating that the MYL7 gene was relatively conser-vative in the evolutionary process.
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