Exploring the Changes of Estrous Markers in Saliva and Serum of Buffalo Based on Untargeted Metabolomics
The problem of inconspicuous estrus in buffaloes is becoming increasingly important in modern livestock production.The aim of this study was to analyse differences in salivary and serum metabolites of buffaloes at different estrus stages using high-throughput untargeted metabolomics.Under the same feeding and management conditions,15 Nyeri Rafi buffaloes were selected,each was injected with 4 mL of chloroprostol,and the day of injection was counted as the 0th day.Saliva and serum samples were collected in the morning before feeding(08:00-11:00 am)during proes-trus(day 1),estrus(day 3)and metestrus(day 6),and they were detected using non-targeted metabolomics.The results showed that different metabolites were present in the saliva and serum of buffaloes at different estrous stages.30 signifi-cantly different metabolites were identified in saliva and 85 significantly different metabolites in serum of buffaloes in the estrus/proestrus;8 significantly different metabolites were identified in saliva and 66 significantly different metabolites in serum of buffaloes in the estrus/metestrus.These different metabolites were mainly enriched in glucagon signalling path-way,pentose phosphate pathway and glycerolipid metabolism pathways.And there was a correlation between salivary me-tabolites and serum metabolites.These salivary metabolites,including D-2-phosphoglyceric acid,L-propionylcarnitine,Leukotriene d4,and 8-iso-prostaglandin a1,may serve as key metabolites for the biomarkers of estrus in buffalo.
万方数据
维普
