Analysis of Antimicrobial Susceptibility Testing,Virulence Genes,and Pathogenicity of Shigella Spp.Isolated from Cattle and Sheep Feces in Taer Reclamation Area,Xinjiang
In order to understand the drug resistance,virulence gene carrying status,and pathogenicity of Shigella from cattle and sheep feces in the Taer reclamation area of Xinjiang,32 Shigella strains(10 Shigella flexneri and 22 Shigella sonnei)isolated and identified from 335 cattle and sheep diarrhea feces were subjected to drug sensitivity testing,resis-tance gene and virulence gene detection,biofilm formation ability,and mouse pathogenicity testing.The results showed that 32 Shigella strains exhibited high levels of resistance to tetracycline,doxycycline,and ampicillin,which were 87.50%,87.50%and 84.38%,respectively.29 strains(90.63%)were identified as multiple drug-resistant(MDR)strains.A total of 15 resistance genes were identified in Shigella,with blaTEM(96.88%)and blaCXT-M(68.75%)exhibiting high de-tection rates.Additionally,14 virulence genes were detected in Shigella with yijp(93.75%),ibeB(90.63%)and ipaBCD(78.13%)showing high detection rates.Some of Shigella species exhibited biofilm-forming capability(2/32,6.25%)and induced resulting in mortality of mice.In summary,the Shigella strains isolated from cattle and sheep in the Taer reclamation area of Xinjiang exhibit heightened levels of antibiotic resistance,harbor multiple resistance genes and virulence genes,demonstrate the capacity for biofilm formation,and possess a degree of pathogenicity posing a poten-tial risk to livestock breeding operations.
万方数据
维普
