Cloning and Bioinformatics Analysis of CXCR2 Gene in Tibetan Sangsang Yak
In order to study the structure,biological function of yak CXCR2 gene,and the mechanism that affects the growth and development of yak,the coding sequence(CDS)of yak CXCR2 gene was cloned by RT-PCR using the adi-pose tissue cDNA of Tibet Sangsang yak as a template.The gene sequence was used for bioinformatics analysis,struc-tural domain prediction and protein physicochemical property prediction,and then RT-qPCR was used for tissue ex-pression analysis.The results showed that the CDS region of the CXCR2 gene of Sangsang yak was 876 bp in length,encoding 291 amino acids.The structural domain prediction of the CXCR2 gene of the yak showed that there was a 7tm_1 structural domain of the Pfam family at position 1-245 of the amino acid sequence of the CXCR2,and the analy-sis of the protein showed that there were five transmembrane structures and no signal peptide region.The moleculor weight was 32 855.55 Da.Its total number of atoms was 4 726.Molecular formula was C1524H2416N392O374S20.Instability index was 29.45.It indicated that the protein was a stable protein with the theoretical isoelectric point of 9.86,fat coeffi-cient of 120.27,and the total average hydrophilicity coefficient of-0.657.It was the hydrophobic proteins with a total of 21 phosphorylation sites.The subcellular localization showed that the CXCR2 gene was mainly distributed in the endo-plasmic reticulum(55.6%),mitochondria(22.2%)and nucleus(22.2%)of the Sangsang yak.The phylogenetic tree showed that the Sangsang yak was the closest to the zebu,and the farthest from the buffalo.
万方数据
维普
