Application of Low Dose CT Combined with Methylation of SHOX2 and RASSF1A in Early Warning of Lung Cancer
Objective To investigate the application value of low dose CT combined with methylation of Ras related region family protein 1A(RASSF1A)and dwarf homeobox gene 2(SHOX2)in early prediction of lung cancer.Methods 90 patients with pulmonary nodule operation in our hospital from January 2021 to January 2023 were selected and divided into benign pulmonary nodule group and lung cancer group according to surgical pathology.Low-dose CT examination and methylation detection of SHOX2 and RASSF1A were performed in both groups before surgery.Kappa index was used to analyze the consistency between the above examination results and surgical pathology.To analyze the diagnostic efficacy of low-dose CT,SHOX2,RASSF1A methylation and serum tumor markers(CEA),neuron-specific enolase(NSE),squamous cell carcinoma antigen(SCC-Ag),and cytokeratin 19 fragment(CYFRA21))in lung cancer.Spearman low-dose CT examination,SHOX2 and RASSF1A methylation were correlated with clinicopathologic features.Results 40 cases,43 cases,46 cases and 58 cases of lung cancer were identified by low dose CT,SHOX2,RASSF1 methylation and their combination,respectively.The consistency of the efficacy of the combination of CT Shox2 and RassF1 in the diagnosis of lung cancer was 0.951 Kappa value.The sensitivity of 96.67％and accuracy of 97.78％were higher than the single diagnostic efficacy of the three methods(P＜0.05).The levels of CEA,SCC,NSE and CYFRA21 in lung cancer patients were higher than those in benign pulmonary nodules(P＜0.05).The AUC of low dose CT combined with SHOX2 and RASSF1 methylation in the diagnosis of lung cancer was 0.983,which was similar to the AUC of 0.933 of the four serum tumor markers.There were significant differences in the detection rate of low-dose CT and methylation positive rate of SHOX2 and RASSF1A among patients with different tumor diameter,clinical stage and histological differentiation(P＜0.05).The detection rate of low-dose CT,the positive rate of methylation of SHOX2 and RASSF1A in lung cancer patients were positively correlated with tumor diameter and clinical stage,but negatively correlated with histological differentiation(P＜0.05).Conclusion Low-dose CT combined with SHOX2 and RASSF1A methylation can be used for early warning of lung cancer,which can be used for early diagnosis and evaluation of the progression of the disease,so as to carry out targeted follow-up treatment and improve the prognosis.
Low-dose CTDwarf Homeobox Gene 2Ras-related Region Family Protein 1ALung CancerSerum Tumor Marker
万方数据
维普
