摘要
目的 探讨定量CT(QCT,quantitative CT)与MRQ-Dixon序列测量小鼠肝脏脂肪含量的可行性研究.方法 本研究采用高脂饮食诱导的肥胖(Diet-induced obesity,DIO)的小鼠动物模型,6周龄C57BL/6小鼠30只,随机分成三组,每组10只,第一组给予普通饲料饲养40周,第二组给予高脂饮食20周,第三组给予高脂饮食40周,2名医师利用QCT和MRQ-Dixon序列对小鼠肝脏脂肪含量进行测量,对QCT测得的肝脏脂肪含量(Fat％Q)和MR Q-Dixon序列测得的肝脏脂肪含量(Fat％M)进行一致性检验和相关性分析.结果 本实验QCT肝脏脂肪含量测量值为 17.6(6.00,28.95)％,MR Q-Dixon序列肝脏脂肪含量测量值为19.6(5.70,29.55)％.两种方法评估脂肪肝严重程度比较,无显著差异(x2=4.000,P=0.216),且一致性较高(Kappa=0.816,P＜0.001);两种方法的测量结果呈正相关(r=0.978,P＜0.01).结论 在定量测量小鼠肝脏脂肪含量方面,定量CT与MR Q-Dixon序列具有较好的一致性和相关性,即可在体定量研究小鼠脂肪肝动物实验.
Abstract
Objective To explore the feasibility study of quantitative CT(QCT)and MR Q-Dixon sequence for measuring liver fat content in mice.Methods In this study,30 6-week-old C57BL/6 mice were randomly divided into three groups with 10 mice in each group.The first group was fed ordinary Diet for 40 weeks,the second group was fed high-fat diet for 20 weeks,and the third group was fed high-fat diet for 40 weeks.Two physicians used QCT and MR Q-dixon sequences to measure the liver Fat content of mice,and conducted consistency test and correlation analysis for the liver Fat content measured by QCT(Fat％Q)and MR Q-dixon(Fat％M).Results The liver fat content measured by QCT and MR Q-Dixon sequences was 17.6(6.00,28.95)％and 19.6(5.70,29.55)％respectively.There was no significant difference between the two methods in evaluating the severity of fatty liver(x 2=4.000,P=0.216),and the consistency was high(Kappa=0.816,P＜0.001).The results of the two methods were positively correlated(r=0.978,P＜0.01).Conclusion In terms of quantitative measurement of liver fat content in mice,quantitative CT has a good agreement and correlation with MR Q-dixon sequence,then it can quantitatively study mouse fatty liver in vivo animal experiments.
基金项目
中国红十字基金医学赋能领航菁英科研项目专项(2022)(XM_LHJY2022_05_07)
安徽医科大学项目(2022xkj043)
NETL
NSTL
万方数据