Feasibility of Measuring Mouse Liver Fat Content Based on MR Q-Dixon Sequence and QCT Sexual Studies
Objective To explore the feasibility study of quantitative CT(QCT)and MR Q-Dixon sequence for measuring liver fat content in mice.Methods In this study,30 6-week-old C57BL/6 mice were randomly divided into three groups with 10 mice in each group.The first group was fed ordinary Diet for 40 weeks,the second group was fed high-fat diet for 20 weeks,and the third group was fed high-fat diet for 40 weeks.Two physicians used QCT and MR Q-dixon sequences to measure the liver Fat content of mice,and conducted consistency test and correlation analysis for the liver Fat content measured by QCT(Fat％Q)and MR Q-dixon(Fat％M).Results The liver fat content measured by QCT and MR Q-Dixon sequences was 17.6(6.00,28.95)％and 19.6(5.70,29.55)％respectively.There was no significant difference between the two methods in evaluating the severity of fatty liver(x 2=4.000,P=0.216),and the consistency was high(Kappa=0.816,P＜0.001).The results of the two methods were positively correlated(r=0.978,P＜0.01).Conclusion In terms of quantitative measurement of liver fat content in mice,quantitative CT has a good agreement and correlation with MR Q-dixon sequence,then it can quantitatively study mouse fatty liver in vivo animal experiments.
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