Analysis on isolation of Yersinia pestis phages in Zhaotong City of Yunnan Province
Objective To investigate whether Yersinia pestis phage are carried in host animals in Zhaotong City,and identify isolated plague phage.Methods In May 2019,host animals were captured by squirrel cage method and 5-meter clamp method in Yongshan,Shuifu and Suijiang counties of Zhaotong.Intestinal samples were collected after identification,Yersinia pestis phages were isolated by double-layer agar plate method,and isolation results were statistically described and analyzed.Results A total of 449 host animals were collected,from which 15 strains of Yersinia pestis phage were separated,with a total isolation rate of 3.34％.The Yersinia pestis phages were isolated in 8 villages,and the highest rates was 16％(4/25)in Yongshan Town.There was no significant difference in the isolation rate of pestis phages among different habitats,sexes and ages.The isolation rate of pestis bacteriophages from different species of host animals was statistically significant.The plaque of the phage is divided into three types,two phages are identified as the Myoviridae family.Conclusion The Yersinia pestis phages exist in Zhaotong,these phages are polymorphic phages and of the Myoviridae family.
Yersinia pestis phageZhaotong CityIsolation result
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