Verification and analysis of urinary iodine measurement by automatic iodine analyzer
Objective To establish a method for detecting urinary iodine by automatic iodine analyzer,to compare it with arsenic cerium catalytic spectrophotometry(manual method),and to analyze the reliability and stability of the method in the range of 0-600 μg/L,and the feasibility of replacing the manual method.Methods Automatic iodine analyzer was used to detect urinary iodine in the range of 0-600 μg/L,and the detection limit,correlation coefficient,precision,accuracy,standard addition recovery rate and other methodological indicators were verified.The urinary iodine content in urine samples was detected at the same time with manual method,and the results were compared.Results In the standard series of 0-600 μg/L concentration range,the correlation coefficient r=0.999 8,the detection limit of the method was 0.87 μg/L,the low,medium and high concentrations of urinary iodine certified reference materials detection results were within the range of the certificate,the relative standard deviation were 2.29％、0.95％、0.60％,respectively.The average recoveries were 99.00％,100.93％and 99.76％.There was no significant difference in the results of 30 urine samples measured simultaneously with the manual method(t=-0.618,P=0.542).Conclusion The automatic iodine analyzer method has high precision and accuracy,wide linear range,high detection efficiency,and can replace the manual method in the range of 0-600 μg/L,which is suitable for popularization and application.
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