Study on association of cancer-related gene polymorphisms with papillary thyroid carcinoma and iodine nutrition
Objective Thyroid cancer is the most common malignant tumor of the thyroid gland,accounting for about 1％of all malignant tumors in the body,and its main histological type is papillary thyroid carcinoma(PTC).The purpose of this study is to investigate the association between cancer-related gene polymorphisms and PTC and iodine nutrition.Methods A total of 613 participants(484 in the PTC group and 129 in the benign tumor group)were included in the case-control study.DNA was extracted,and 10 gene loci were selected from TSHR,PDE8B,BRAF and TPO to analyze their polymorphisms by polymerase chain reaction(PCR).In this study,PTCs were grouped according to the disease type,number of lesions,lymph node metastases,different urinary iodine and different serum iodine concentrations to study the association of gene polymorphisms with above factors.Results The proportion of BRAF rs3748093 mutant(AT+AA)in the PTC group was significantly higher than that in the benign tumor group.There were no statistically significant differences between the two groups for the remaining genotypes.In patients with PTC,group by number of lesions,TSHR rs4903961 mutant(CG+CC)accounted for a higher proportion of single lesions than multiplelesions;There was no significant association between the other genotypes and the occurrence and development of PTC;When the urine iodine＜100 µg/L,the homozygous carrier rate of PDE8B rs7714529 was significantly higher than that of the other urinary iodine groups;When urinary iodine＞300 pg/L,the homozygous carrier rate of BRAF rs17623204 and TPO rs732608 mutant(including heterozygous and homozygous)were significantly higher than that of the other urinary iodine groups;When serum iodine＜45 pg/L,the carrier rate of BRAF rs17623204 heterozygous(AT)was significantly higher than that of the other serum iodine groups;The remaining genotypes were not significantly associated with iodine nutrient levels.Conclusion BRAF rs3748093 AT genotype is associated with PTC susceptibility;TSHR rs4903961 mutant(CG+CC)is associated with the development of PTC;Homozygous for PDE8B rs7714529(AA+GG)was associated with low urinary iodine;BRAF rs17623204 homozygous(TT+AA)and TPO rs732608 mutant(CT+TT)were associated with high urinary iodine;BRAF rs17623204 heterozygous is associated with low serum iodine.
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