Effects of Spraying Methyl Jasmonate at Seedling Stage on Aroma of Fragrant Rice Xiangyaxiangzhan
Methyl jasmonate is known to enhance plant stress resistance,however,its specific effects on the accumulation of 2-acetyl-1-pyrroline(2-AP)in fragrant rice have not been extensively investigated.In this experiment,Xiangyaxiangzhan,a fragrant rice variety,was selected as the test material.Three concentrations of methyl jasmonate(0.25,0.50,and 1.00 mmol/L)were applied during the seedling stage to examine the impact on the synthesis and accumulation of 2-AP,as well as the levels of aroma precursors,activity of aroma-related enzymes,regulation of aroma-related gene expression,changes in enzyme activities related to stress resistance,and seedling quality.The results showed that compared to the control(CK),spraying appropriate concentrations of methyl jasmonate(0.50-1.00 mmol/L)increased expression of DAO4,OA T,and ProDH in fragrant rice seedlings,accompanied by an elevation in 2-AP content.Furthermore,spraying methyl jasmonate increased the levels of aroma synthesis precursors including 1-pyrroline,methylglyoxal,and P5C.This resulted in an improvement in the quality of fragrant rice seedlings,an increase in the activity of stress-resistant enzymes,and enhanced antioxidant capacity.