Identification of Favorable Genes and Breeding Utilization of Introgression Line Guihui 1561 Originated from Oryza minuta
Exploring beneficial genes from wild rice for genetic improvement of cultivated rice is a hot topic in current rice breeding research.Guihui 1561,an introgression line originated from Oryza minuta,was obtained by distant hybridization.Through agronomic trait examination,combining ability determination,and rice planthopper resistance identification,we confirmed that Guihui 1561 has large grain(32.2 g),long panicle(28.3 cm),high quality,good combining ability,and resistance to both brown planthopper(resistance level 2.5)and white back planthopper(resistance level 2.9).It has high breeding value.Subsequently,the beneficial genes from Guihui 1561 were mapped,and linkage molecular markers for four major genes,PL9,GS12,Bph31(t),and Wbph9(t),were developed,using the recombinant inbred lines and near-isogenic lines constructed from Gui 1025/Guihui 1561.The beneficial genes of Guihui 1561 were introduced into multiple backbone parents using molecular marker-assisted breeding technology,and 9 new high quality and multi-resistance restorer lines were created.Furthermore,11 dominant hybrid rice combinations were bred using Guihui 1561 and its derivative lines as restorer lines,among which Huazheyou 1561 and Hanyou 711 have been approved by the Crop Variety Certification Committee of Guangxi Zhuang Autonomous Region.