Analysis of Variation Types of BADH2 Aroma Genes and Detection of Rice Blast Resistance Genes in 80 Aromatic Rice Materials
Using six aroma gene variation types and five rice blast broad-spectrum resistance gene functional markers,we detected the Badh2 aroma gene variant types and rice blast resistance genes in 80 aromatic rice materials.At the same time,we also counted the rice blast identification results of the approved varieties in rice regional trial,and identified the aroma and rice blast phenotype.The results showed that all 80 aromatic rice materials possessed aroma,with 7 samples(accounting for 8.75％)having a 803 bp deletion mutation in exons 4 to 5,and 4 samples(accounting for 5.00％)containing two types of variants with a 3 bp deletion in the 5'UTR region and an 8 bp deletion and 3 bp mutation in exon 7.The remaining 69 samples(accounting for 86.25％)had the variant type of 8 bp deletion and 3 bp mutation in exon 7.Among the 80 aromatic rice materials,the numbers containing the Pita,Pi2,Pi9,Pigm,and Pi1 resistance genes were 57(71.25％),12(15.00％),4(5.00％),1(1.25％),and 1(1.25％),respectively.There are 9 materials containing both Pi2 and Pita(accounting for 11.25％),1 material containing both Pigm and Pita(accounting for 1.25％),and 1 material containing both Pi1 and Pita(accounting for 1.25％).The proportion of materials carrying two resistance genes simultaneously exhibiting disease resistance is higher than that of materials carrying only one resistance gene and those without resistance genes.The research results provide a basis for the application of aromatic rice materials and the pyramiding of rice blast resistance genes,as well as for molecular marker-assisted breeding of aromatic rice.
万方数据
维普
